Stimulation of nerve fiber growth from cultured sympathetic explants.
Stimulation of nerve fiber growth from cultured sympathetic explants.
复制标题
刺激培养的交感神经外植体的神经纤维生长。
DOI：
10.1139/y74-109
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发表时间：
1974
影响因子：
2.1
通讯作者：
R. H. Hall
中科院分区：
文献类型：
--
作者：
A. Recklies;M. Rathbone;R. H. Hall
N6，O2′-二丁酰腺苷-3 ′，5 ′-环磷酸（DBcAMP）和前列腺素E1（PGE 1）均能促进神经母细胞瘤细胞的成熟和分化。这种作用可通过几种磷酸二酯酶抑制剂增强。在体外培养的鸡胚交感神经节中，我们发现，在培养液中，洋地黄素E1和E2以及环磷酸腺苷（cAMP）和环磷酸腺苷（DBcAMP）均能诱导神经突起的生长。DBcAMP的影响也诱发丁酸钠的培养基中加入。当在4 mM茶碱存在下使用这些化合物时，神经突生长几乎不超过在对照培养物中观察到的生长。 茶碱也减少了在NGF存在下获得的神经纤维生长的量。因此，它似乎可能是theophyl...
Both N6,O2′-dibutyryl adenosine-3′,5′-cyclic monophosphate (DBcAMP) and prostaglandin E1 (PGE1) stimulate maturation and differentiation of neuroblastoma cells in tissue culture. This effect is potentiated by several phosphodiesterase inhibitors. DBcAMP and adenosine-3′,5′-cyclic monophosphate (cAMP) have also been reported to mimic the effects of nerve growth factor (NGF) on dorsal root ganglia but not on sympathetic ganglia in stimulating neurite outgrowth.We found that prostaglandins E1 and E2 as well as cAMP and DBcAMP elicit neurite outgrowth from chick embryo sympathetic ganglia incubated in microtest tissue culture plates with a liquid medium. The effects of DBcAMP were also evoked by the addition of sodium butyrate to the culture medium. When these compounds were used in the presence of 4 mM theophylline the neurite growth barely exceeded that observed in control cultures. Theophylline also reduced the amount of nerve fiber growth obtained in the presence of NGF. Thus it seems likely that theophyl...