Synthesis of two forms of apolipoprotein B by cultured rat hepatocytes.
Synthesis of two forms of apolipoprotein B by cultured rat hepatocytes.
复制标题
培养的大鼠肝细胞合成两种形式的载脂蛋白 B。
DOI：
10.1016/0006-291x(81)91800-3
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
Graham,P
中科院分区：
文献类型：
--
作者：
Bell-Quint,J;Forte,T;Graham,P
培养的大鼠肝细胞用于证明肝脏可以合成两种形式的载脂蛋白B。载脂蛋白B的圆盘凝胶电泳分离表明，肝细胞低密度脂蛋白主要含有载脂蛋白B，其表观分子量为345，000 ± 5，055。相比之下，肝细胞极低密度脂蛋白的主要载脂蛋白B组分是分子量为242，000 ± 2，720的变体形式。与血浆HDL不同，肝细胞高密度脂蛋白还含有表观分子量为244，000 ± 2，742的载脂蛋白B。[3H]亮氨酸掺入肝细胞载脂蛋白B组分提示从头合成。
Cultured rat hepatocytes were used to demonstrate that the liver can synthesize two forms of apolipoprotein B. Separation of apolipoprotein B by disc gel electrophoresis indicated that hepatocyte low density lipoprotein contains predominantly apolipoprotein B with an apparent molecular weight of 345,000 ± 5,055. In contrast, the major apolipoprotein B component of hepatocyte very low density lipoprotein is a variant form with a molecular weight of 242,000 ± 2,720. Hepatocyte high density lipoprotein, unlike plasma HDL, also contains apolipoprotein B with an apparent molecular weight of 244,000 ± 2,742. Incorporation of [3H] leucine into hepatocyte apolipoprotein B components suggested de novo synthesis.