Cytochalasin. D, a tropical fungal metabolite, inhibits CT26 tumor growth and angiogenesis
Cytochalasin. D, a tropical fungal metabolite, inhibits CT26 tumor growth and angiogenesis
复制标题
细胞松弛素。
DOI：
10.1016/s1995-7645(12)60019-4
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发表时间：
2012-03-01
影响因子：
3.1
通讯作者：
Tan, Guang Hong
中科院分区：
文献类型：
--
作者：
Huang, Feng Ying;Li, Yue Nan;Tan, Guang Hong
目的：研究细胞松弛素D是否具有抗肿瘤活性。研究方法：体外培养人结肠癌细胞系Murnie CT 26，以细胞松弛素D为细胞毒药物，通过MTT法和TUNEL法检测细胞松弛素D对CT 26细胞增殖的抑制作用和诱导细胞凋亡的作用。观察建立小鼠CT 26肿瘤模型。肿瘤生长和生存时间。采用免疫组化法检测肿瘤组织中微血管密度。另外。藻酸盐包封的肿瘤细胞测定用于定量体内肿瘤血管生成。结果如下：细胞松弛素D以时间和密切依赖的方式抑制CT 26肿瘤细胞的增殖，并诱导CT 26细胞凋亡，几乎达到阳性对照核酸酶诱导的水平。细胞松弛素D体内治疗的最佳有效剂量为50 mg/kg左右。葡萄酒中的细胞松弛素治疗显著抑制了CT 26肿瘤听力小鼠的肿瘤生长并延长了存活时间。免疫组化和海藻酸钠包埋实验结果表明，细胞松弛素D能有效抑制肿瘤血管生成。结论：细胞松弛素D可能通过抑制细胞增殖、诱导细胞凋亡和抑制肿瘤血管生成来抑制CT 26肿瘤生长。
Objective: To investigate whether cytochalasin D can induce antitumor activities in a tumor model. Methods: Murnie CT26 colorectal carcinoma cells were cultured in vitro and cytochalasin D was used as a cytotoxic agent in rimed its capabilities of inhibiting CT26 cell proliferation and inducing cell apoptosis by MTT and it TUNEL-based apoptosis assay. Murine CT26 tumor model was established in observe. the tumor growth and survival time. Tumor tissues were used to detect the microvessel density by immunohistochemistry. In addition. alginate encapsulated tumor cell assay was used to quantify the tumor angiogenesis in vivo. Results: Cytochalasin D inhibited CT26 tumor cell proliferation in lime and close dependent manner and induced significant CT26 cell apoptosis, which almost reached the level induced by the positive control nuclease. The optimum effective (lose of cytochalasin D for in vivo therapy was about 50 mg/kg. Cytochalasin in Vino treatment significantly inhibited tumor growth and prolonged the survival times in CT26 tumor-hearing mice. The results of immunohistochemistry analysis and alginate encapsulation assay indicated that the cytochalasin D could effectively inhibited tumor angiogenesis. Conclusions: Cytochalasin D inhibits CT26 tumor growth potentially through inhibition of cell proliferation, induction of cell apoptosis and suppression of tumor angiogenesis.