API2-MALT1 chimeric transcripts involved in mucosa-associated lymphoid tissue type lymphoma predict heterogeneous products
API2-MALT1 chimeric transcripts involved in mucosa-associated lymphoid tissue type lymphoma predict heterogeneous products
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DOI：
10.1016/s0002-9440(10)64948-6
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发表时间：
2000-03-01
影响因子：
6
通讯作者：
Seto, M
中科院分区：
文献类型：
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作者：
Motegi, M;Yonezumi, M;Seto, M
低度恶性B细胞淋巴瘤分子生物学研究的最新进展表明，位于11 q21的API 2和位于18 q21的新基因MALT 1参与了t（11;18）（q21;q21），这是粘膜相关淋巴组织（MALT）型淋巴瘤的特征性染色体畸变。我们建立了一种逆转录-聚合酶链反应（RT-PCR）方法，并对22例MALT淋巴瘤进行了分析。所有5例显示具有t（11;18）（q21;q21）的病例均显示API 2-MALT 1嵌合转录物的特异性扩增。在其余17例细胞遗传学数据完全不可用的病例中，3例证实存在融合转录本，表明本系列中MALT淋巴瘤病例的显著百分比似乎具有t（11;18）。在这些病例中均观察到单个碎片，但大小因病例而异。测序分析表明，API 2有两个断点，MALT 1有三个断点，所有的融合转录本都在读码框内。在这些结果的基础上，四种嵌合蛋白可以预测本系列。因此，这里使用的RT-PCR检测应作为一个有效的分子工具，了解分子发病机制和MALT淋巴瘤的API 2-MALT 1的临床意义。
Recent progress in molecular analysis of low-grade B cell lymphoma has revealed that API2 at 11q21 and a novel gene, MALT1 at 18q21, are involved in t(11;18)(q21;q21), a characteristic chromosome aberration for mucosa-associated lymphoid tissue (MALT) type lymphoma. We describe here the establishment of a reverse transcription-polymerase chain reaction (RT-PCR) assay that me used to analyze 22 cases of MALT lymphoma. All five cases that were shown to possess t(11;18)(q21;q21) showed the specific amplification of API2-MALT1 chimeric transcripts. Of the remaining 17 cases for which cytogenetic data mere not available, three cases demonstrated the presence of fusion transcripts, indicating that a significant percentage of MALT lymphoma cases of the present series appeared to possess t(11;18). A single fragment was observed in each of these cases, but the size varied from case to case. Sequencing analysis revealed that there are two breakpoints in API2 and three in MALT1, and that all of the fusion transcripts are in-frame. On the basis of these results, four kinds of chimeric proteins can be predicted for the present series. Thus, the RT-PCR assay used here should serve as an effective molecular tool for understanding molecular pathogenesis and the clinical significance of API2-MALT1 for MALT lymphomas.