Zbtb21 is required for the anterior-posterior patterning of neural tissue in the early Xenopus embryo
Zbtb21 is required for the anterior-posterior patterning of neural tissue in the early Xenopus embryo
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Zbtb21 对于早期非洲爪蟾胚胎神经组织的前后模式是必需的
DOI：
10.1016/j.bbrc.2022.09.048
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Suzuki A.
中科院分区：
文献类型：
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作者：
Takebayashi-Suzuki K;Uchida M;Suzuki A.
脊椎动物的身体分别通过 BMP 和 Wnt 通路沿着背腹 (DV) 轴和前后 (AP) 轴组织。我们之前报道过 XenopusZbtb14 通过抑制 BMP 信号传导促进外胚层的背侧化（神经诱导），并通过激活 Wnt 信号传导使神经组织后侧化，从而协调早期发育过程中 DV 和 AP 轴的模式。尽管有报道称人类ZBTB21与ZBTB14结合并参与培养的哺乳动物细胞中的基因表达，但Zbtb21在早期胚胎发育中的功能仍不清楚。在这里，我们发现非洲爪蟾Zbtb21在早期非洲爪蟾胚胎AP轴的形成中起着至关重要的作用。zbtb21和zbtb14在原肠胚形成过程中在胚胎的背侧区域共表达。在外胚层外植体中同时过表达 Zbtb21 和 Zbtb14 可增强 Zbtb14 的神经诱导活性。此外，敲低实验表明，Zbtb21对于后神经组织的形成和前神经发育的抑制是必需的。总的来说，这些结果表明，Zbtb21 与 Zbtb14 合作，在早期爪蟾胚胎发生过程中的 AP 模式中发挥着至关重要的作用。
The vertebrate body is organized along the dorsal-ventral (DV) and anterior-posterior (AP) axes by the BMP and Wnt pathways, respectively. We previously reported thatXenopusZbtb14 promotes dorsalization (neural induction) of ectoderm by inhibiting BMP signaling and also posteriorizes neural tissue by activating Wnt signaling, thereby coordinating the patterning of the DV and AP axes during early development. Although it has been reported that human ZBTB21 binds to ZBTB14 and is involved in gene expression in cultured mammalian cells, the function of Zbtb21 in early embryonic development remains unknown. Here, we show thatXenopusZbtb21 plays an essential role in AP axis formation in the earlyXenopusembryo.zbtb21andzbtb14are co-expressed in the dorsal region of embryos during gastrulation. Simultaneous overexpression of Zbtb21 and Zbtb14 in ectodermal explants enhances the neural-inducing activity of Zbtb14. Moreover, knockdown experiments showed that Zbtb21 is required for the formation of posterior neural tissue and the suppression of anterior neural development. Collectively, these results suggest that in cooperation with Zbtb14, Zbtb21 has a crucial function in AP patterning during earlyXenopusembryogenesis.