Functional conservation of RNA polymerase II in fission and budding yeasts
Functional conservation of RNA polymerase II in fission and budding yeasts
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DOI：
10.1006/jmbi.1999.3399
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发表时间：
2000-02-04
影响因子：
5.6
通讯作者：
Thuriaux, P
中科院分区：
文献类型：
--
作者：
Shpakovski, GV;Gadal, O;Thuriaux, P
互补DNA的12个亚基的裂殖酵母（裂殖酵母）RNA聚合酶II的表达从强启动子在酿酒酵母和测试异源特异性互补通过监测其能力，以取代在体内的无效，相应的宿主基因的突变体。Rpb 1和Rpb 2，两个最大的亚基和Rpb 8，一个小的亚基共享的所有三个聚合酶，未能支持在酿酒酵母中的生长。其余9个亚基都精通异源特异性互补，并导致在大多数情况下，野生型水平的增长。然而，两个α样亚基（Rpb3和Rpb11）不支持在高温（37摄氏度）或低温（25摄氏度）下生长。在Rpb3的情况下，生长恢复增加宿主Rpb11或Rpb10亚基的基因剂量，证实了这三个亚基之间的密切遗传相互作用的证据。(C)北京大学出版社.
The complementary DNAs of the 12 subunits of fission yeast (Schizosaccharomyces pombe) RNA polymerase II were expressed from strong promoters in Saccharomyces cerevisine and tested for heterospecific complementation by monitoring their ability to replace in vivo the null, mutants of the corresponding host genes. Rpb1 and Rpb2, the two largest subunits and Rpb8, a small subunit shared by all three polymerases, failed to support growth in S, cerevisiae. The remaining nine subunits were all proficient for heterospecific complementation and led in most cases to a wild-type level of growth. The two alpha-like subunits (Rpb3 and Rpb11), however, did not support growth at high (37 degrees C) or low (25 degrees C) temperatures. Ln the case of Rpb3, growth was restored by increasing the gene dosage of the host Rpb11 or Rpb10 subunits, confirming previous evidence of a close genetic interaction between these three subunits. (C) 2000 Academic Press.