Rapid Visualization and Large-Scale Profiling of Bacterial Lipoproteins with Chemical Reporters
Rapid Visualization and Large-Scale Profiling of Bacterial Lipoproteins with Chemical Reporters
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DOI：
10.1021/ja101387b
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发表时间：
2010-08-11
影响因子：
15
通讯作者：
Hang, Howard C.
中科院分区：
文献类型：
--
作者：
Rangan, Kavita J.;Yang, Yu-Ying;Hang, Howard C.
脂蛋白是细菌中一种很大程度上未被表征的蛋白质。在这项研究中，用脂肪酸化学报告分子对细菌进行代谢标记，可以快速分析脂质修饰的蛋白质。我们在大肠杆菌中鉴定了许多候选脂蛋白，并在YjgF上检测到一种新的修饰。这种化学方法应有助于脂蛋白的未来表征。
Lipoproteins are a largely uncharacterized class of proteins in bacteria. In this study, metabolic labeling of bacteria with fatty acid chemical reporters allowed rapid profiling of lipid-modified proteins. We identified many candidate lipoproteins in Escherichia coli and detected a novel modification on YjgF. This chemical approach should facilitate future characterization of lipoproteins.