Short-term culture of peritoneum explants confirms attachment of endometrium to intact peritoneal mesothelium
Short-term culture of peritoneum explants confirms attachment of endometrium to intact peritoneal mesothelium
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DOI：
10.1016/s0015-0282(00)01699-x
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发表时间：
2001-02-01
影响因子：
6.7
通讯作者：
Schenken, RS
中科院分区：
文献类型：
--
作者：
Witz, CA;Thomas, MR;Schenken, RS
目的：评价子宫内膜与腹膜的初始粘附性。设计：采用光镜和激光共聚焦扫描显微镜、免疫组织化学和透射电镜进行描述性研究。设定：主要结果测量：腹膜外植体（n = 20），从4名患者制备，与机械分散的增殖或分泌子宫内膜一起培养1小时。取同一患者的腹膜与子宫内膜进行培养。结果：20例移植物中17例子宫内膜与完整的间皮层粘连。在任何附着部位都没有跨间皮入侵的证据。虽然在大多数情况下，子宫内膜通过子宫内膜间质粘附于间皮，但子宫内膜上皮-间皮附着的部位很多。共聚焦激光扫描显微镜显示，在子宫内膜种植部位下方有完整的细胞角蛋白阳性细胞单层。透射电子显微镜显示完整的，可行的，mesothelium细胞以下的网站attachment. Conclusions（S）：这项研究表明，子宫内膜迅速粘附到完整的腹膜mesothelium。此外，本研究表明，子宫内膜上皮细胞。以及基质，可以附着在间皮上。需要进一步的研究，表征间皮细胞粘附的机制。（C）2001年由美国生殖医学会。
Objective: To evaluate the initial adhesion of endometrium to the peritoneum.Design: Descriptive study using light and confocal laser-scanning microscopy, immunohistochemistry, and transmission electron microscopy. Setting: University-based laboratory.Patient(s): Women without endometriosis undergoing surgery for benign conditions.Intervention(s): None.Main Outcome Measure(s): Explants of peritoneum (n = 20), prepared from four patients, were cultured for I hour with mechanically dispersed proliferative or secretory endometrium. Peritoneum was cultured with endometrium from the same patient. Specimens were fixed and serially sectioned for hematoxylin and eosin stain, immunohistochemistry using an anti-cytokeratin monoclonal antibody, and transmission electron microscopy.Result(s): In 17 of 20 explants, endometrium was adherent to intact mesothelium. There was no evidence of transmesothelial invasion at any sites of attachment. Although in most cases endometrium was adherent to mesothelium via endometrial stroma, there were many sites of endometrial epithelium-mesothelium attachment. Confocal laser scanning microscopy demonstrated an intact monolayer of cytokeratin-positive cells below the sites of endometrial implantation. Transmission electron microscopy demonstrated intact, viable, mesothelial cells below sites of attachment.Conclusion(s): This study demonstrates that endometrium rapidly adheres to intact peritoneal mesothelium. In addition, this study demonstrates that endometrial epithelial cells. as well as stroma, can attach to mesothelium. Further studies are needed that characterize the mechanism of endometrial-mesothelial cell adhesion. (C)2001 by American Society for Reproductive Medicine.