Exercise induces region-specific remodeling of astrocyte morphology and reactive astrocyte gene expression patterns in male mice
Exercise induces region-specific remodeling of astrocyte morphology and reactive astrocyte gene expression patterns in male mice
复制标题
DOI：
10.1002/jnr.24430
复制
发表时间：
2019-09-01
影响因子：
4.2
通讯作者：
Jakowec, Michael W.
中科院分区：
文献类型：
--
作者：
Lundquist, Adam J.;Parizher, Jacqueline;Jakowec, Michael W.
星形胶质细胞是突触传递和神经可塑性许多方面的重要介质。运动已被证明可以诱导神经可塑性和突触重塑，例如通过介导神经退行性变动物模型中的神经康复。然而，运动对星形胶质细胞功能的影响以及这些变化如何与神经可塑性相关仍不清楚。在这里，我们通过雄性小鼠的 GFAP 和 SOX9 免疫组织化学和形态学分析测量表明，运动以运动和区域依赖性方式重塑星形胶质细胞。此外，对反应性星形胶质细胞基因表达的 qRT-PCR 分析显示，已知运动激活的大脑区域会因运动而升高。总而言之，这些数据表明运动积极改变星形胶质细胞形态并驱动星形胶质细胞基因表达的变化，并表明星形胶质细胞可能是运动诱导的神经可塑性和神经康复的核心组成部分。
Astrocytes are essential mediators of many aspects of synaptic transmission and neuroplasticity. Exercise has been demonstrated to induce neuroplasticity and synaptic remodeling, such as through mediating neurorehabilitation in animal models of neurodegeneration. However, the effects of exercise on astrocytic function, and how such changes may be relevant to neuroplasticity remain unclear. Here, we show that exercise remodels astrocytes in an exercise- and region-dependent manner as measured by GFAP and SOX9 immunohistochemistry and morphological analysis in male mice. Additionally, qRT-PCR analysis of reactive astrocyte gene expression showed an exercise-induced elevation in brain regions known to be activated by exercise. Taken together, these data demonstrate that exercise actively modifies astrocyte morphology and drives changes in astrocyte gene expression and suggest that astrocytes may be a central component to exercise-induced neuroplasticity and neurorehabilitation.