phoR sequences as a phylogenetic marker to differentiate the species in the Bacillus subtilis group
phoR sequences as a phylogenetic marker to differentiate the species in the Bacillus subtilis group
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phoR 序列作为系统发育标记来区分枯草芽孢杆菌组中的物种
DOI：
10.1139/w2012-106
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发表时间：
2012-11-01
影响因子：
2.8
通讯作者：
Ma, Ping
中科院分区：
文献类型：
--
作者：
Guo, Qinggang;Li, Shezeng;Ma, Ping
枯草芽孢杆菌及其近缘种在形态特征和16S rDNA序列上难以区分。在这项研究中，测试了部分phor序列，以确定物种在枯草杆菌群中的系统发育关系。根据枯草芽孢杆菌phor基因相对保守的核苷酸序列和枯草芽孢杆菌的连锁基因Phop，设计了简并引物。从枯草芽孢杆菌群中代表6个种的菌株中扩增出1100bp的pho片段。根据测序片段，明确区分了这6个种的26个模式菌株。在种内水平上，PHO序列的相似性为90%~100%，而在种间水平上，PHOR序列的相似性为32.8%~75%。与gyrB序列相比，phor序列表现出更大的差异，尤其是在种间水平上。因此，phor序列可能是枯草杆菌属物种系统发育和分类分析的一种有效的替代标记。对23株未驯化的芽孢杆菌进行了phor和gyrB序列的鉴定和系统发育分析。23株菌株可清晰地划分为4个不同的类群，其中枯草芽孢杆菌10个，莫杰文氏芽孢杆菌3个，大西洋芽孢杆菌2个，淀粉溶解芽孢杆菌8个。
Bacillus subtilis and its closely related species are indistinguishable from one another by morphological characteristics and 16S rDNA sequences. In this study, the partial phoR sequence was tested to determine the phylogenetic relationship of species in the B. subtilis group. Degenerate primers were developed according to the relatively conserved nucleotide sequences of phoR and the linked gene phoP in the B. subtilis group. The primers amplified a 1100 bp phoR fragment from strains representative of 6 species in the B. subtilis group. Based on the sequenced fragments, 26 type strains comprising these 6 species were clearly distinguished. At the intraspecies level, the phoR sequence similarities were 90%-100%, but at the interspecies level, the phoR sequence similarities were 32.8%-75%. Compared with the gyrB sequence, the phoR sequences showed a larger divergence especially at the interspecies levels. Therefore, the phoR sequence may be an efficient alternative marker for phylogenetic and taxonomic analysis of species in the B. subtilis group. Twenty-three Bacillus undomesticated isolates were tested for identification and phylogenetic analysis based on the phoR and gyrB sequences. The 23 isolates could be clearly delineated into 4 distinct groups, 10 as B. subtilis, 3 as B. mojavensis, 2 as B. atrophaeus, and 8 as B. amyloliquefaciens.