Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus furiosus
Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus furiosus
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DOI：
10.1016/j.carres.2014.01.021
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发表时间：
2014-03-31
影响因子：
3.1
通讯作者：
Kohda, Daisuke
中科院分区：
文献类型：
--
作者：
Fujinami, Daisuke;Matsumoto, Masaki;Kohda, Daisuke
通过体外低聚糖转移反应，利用含有来自超嗜热古细菌——狂热焦球菌（Pyrococcus furiosus）的低聚糖转移酶的膜组分，产生了连接在结构确定的肽中的天冬酰胺残基上的低聚糖链。通过糖分析、核磁共振光谱和质谱分析对n -聚糖的化学结构进行了鉴定，揭示了n -聚糖的结构。α - d - manpnac -(1 -> 4)- α - d - glcap -(1 -> 3)- α - d - manp -(1 -> 2)- α - d - manp -(1 -> 3)- β - d - galpnac - asm 3 2向上箭头向上箭头1 - d - xylp β - d - xylp缺少一个或两个木糖残基的较短的聚糖结构也被P. furiosus寡糖转移酶转移。已知古细菌n -聚糖表现出高度的结构变化。P. furiosus n -聚糖的结构是新颖而独特的。本研究结果将有助于对该类低聚糖转移酶的结构和功能的研究。(C) 2014 Elsevier Ltd.版权所有。
An oligosaccharide chain attached to the asparagine residue in a structurally defined peptide was produced by an in vitro oligosaccharide-transfer reaction, using membrane fractions that contained the oligosaccharyltransferase from the hyperthermophilic archaeon, Pyrococcus furiosus. The chemical structure of the N-glycan was elucidated by sugar analysis, NMR spectroscopy, and MS spectrometry, which revealed the structure.alpha-D-ManpNAc-(1 -> 4)-alpha-D-GlcAp-(1 -> 3)-alpha-D-Manp-(1 -> 2)-alpha-D-Manp-(1 -> 3)-beta-D-GalpNAc-Asm 3 2 up arrow up arrow 1 1 beta-D-Xylp beta-D-XylpThe shorter glycan structures lacking one or two xylose residues were also transferred by the P. furiosus oligosaccharyltransferase. The archaeal N-glycans are known to exhibit a high degree of structural variation. The structure of the P. furiosus N-glycan is novel and unique. The present data will be useful for structural and functional studies of the P. furiosus oligosaccharyltransferase. (C) 2014 Elsevier Ltd. All rights reserved.