Naked eye detection of mutagenic DNA photodimers using gold nanoparticles.
Naked eye detection of mutagenic DNA photodimers using gold nanoparticles.
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DOI：
10.1016/j.bios.2010.11.019
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发表时间：
2011-01
影响因子：
12.6
通讯作者：
J. Kim;B. Chung
中科院分区：
文献类型：
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作者：
J. Kim;B. Chung
我们开发了一种使用金纳米颗粒通过肉眼检测致突变 DNA 光二聚体的方法。金纳米颗粒在高离子强度溶液中的稳定性是通过物理吸附在 AuNP 上的直单链 DNA 来维持的。然而，我们发现，由于紫外线照射的 DNA 发生了构象变化，因此金纳米粒子对紫外线照射的 DNA 的吸附性较差。多二聚体形成引起的 DNA 结构的累积变形引发了与紫外线照射的 DNA 混合的金纳米粒子的聚集，从而使混合物的颜色从红色变为紫色，从而可以通过肉眼对 DNA 光二聚体进行比色检测。 UVB 照射下没有碎片质量和活性氧产生，证实金纳米粒子的聚集完全归因于 UV 照射 DNA 的累积变形。金纳米粒子聚集的程度取决于 UVB 照射时间和 UV 照射寡核苷酸的碱基组成。此外，我们还成功演示了如何通过应用这种新方法在短短 10 分钟内并行视觉鉴定化合物的光敏效应。由于我们的方法不需要任何化学或生化处理或特殊仪器来纯化和鉴定DNA光损伤，因此它应该为紫外线诱导突变或致癌DNA二聚体的研究提供可行的工具，并加速大量候选药物的筛选。
We developed a method to detect mutagenic DNA photodimers by the naked eye using gold nanoparticles. The stability of gold nanoparticles in a high ionic strength solution is maintained by straight ssDNA adsorbed physically on the AuNPs. However, we found that UV-irradiated DNA was less adsorptive onto gold nanoparticles because of a conformational change of UV-irradiated DNA. The accumulated deformation of the DNA structure by multiple-dimer formation triggered aggregation of the gold nanoparticles mixed with the UV-irradiated DNA and thus red to purple color changes of the mixture, which allowed colorimetric detection of the DNA photodimers by the naked eye. No fragmented mass and reactive oxygen species production under the UVB irradiation confirmed that the aggregation of gold nanoparticles was solely attributed to the accumulated deformation of the UV irradiated DNA. The degree of gold nanoparticles-aggregation was dependent on the UVB irradiated time and base compositions of the UV-irradiated oligonucleotides. In addition, we successfully demonstrated how to visually qualify the photosensitizing effect of chemical compounds in parallel within only 10min by applying this new method. Since our method does not require any chemical or biochemical treatments or special instruments for purifying and qualifying the DNA photolesions, it should provide a feasible tool for the studies of the UV-induced mutagenic or carcinogenic DNA dimers and accelerate screening of a large number of drug candidates.