Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach
Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach
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DOI：
10.1007/pl00013817
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发表时间：
1999-12-01
期刊：
影响因子：
--
通讯作者：
Hinnen, A
中科院分区：
文献类型：
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作者：
Entian, KD;Schuster, T;Hinnen, A
在一个系统的方法来研究酿酒酵母基因的未知功能，150缺失突变体（1双，149单突变体）的构建和表型分析。在所有被检测的基因中，有20%是必需的。对活的缺失突变体进行20种不同的测试系统，从高通量到高度特异性的测试系统。表型获得了三分之二的突变体测试。在研究过程中，其他人描述了26个基因的突变体。对于这些报告的数据是根据我们的结果。令人惊讶的是，在我们的测试中发现了7个基因的额外的、意想不到的表型。这表明，这里提出的分析类型提供了更完整的基因功能描述。
In a systematic approach to the study of Saccharomyces cerevisiae genes of unknown function, 150 deletion mutants were constructed (1 double, 149 single mutants) and phenotypically analysed. Twenty percent of all genes examined were essential. The viable deletion mutants were subjected to 20 different test systems, ranging from high throughput to highly specific test systems. Phenotypes were obtained for two-thirds of the mutants tested. During the course of this investigation, mutants for 26 of the genes were described by others. For is of these the reported data were in accordance with our results. Surprisingly, for seven genes, additional, unexpected phenotypes were found in our tests. This suggests that the type of analysis presented here provides a more complete description of gene function.