EFFECT OF OXYGEN-TENSION ON ALDOLASES OF MYCOBACTERIUM-TUBERCULOSIS H37RV
EFFECT OF OXYGEN-TENSION ON ALDOLASES OF MYCOBACTERIUM-TUBERCULOSIS H37RV
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DOI：
10.1016/0014-5793(74)80812-4
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发表时间：
1974-01-01
期刊：
影响因子：
3.5
通讯作者：
VENKITAS.TA
中科院分区：
文献类型：
--
作者：
BAI, NJ;PAI, MR;VENKITAS.TA
3. Results M. tuberculosis H3, Rv (NCTC 7416) was grown at 37 C for 3 weeks in Youmans and Karlson medium [4] containing 2% glycerol, either in a fermentor or as surface culture.Cell-free extracts were prepared by disruption of a 20% cell suspension, in 0.05 M phosphate buffer, pH 6, containing 1 mM 2-mercaptoethanol, using a Raytheon sonic oscillator, 10 KHz for 30 min and subsequent centrifugation at 20 OOOg for 30 min. After precipitation of the nucleic acids with protamine
