Transfected mouse melanoma lines that express various levels of human melanoma-associated antigen p97.
Transfected mouse melanoma lines that express various levels of human melanoma-associated antigen p97.
复制标题
表达不同水平的人类黑色素瘤相关抗原 p97 的转染小鼠黑色素瘤系。
DOI：
10.1093/jnci/81.6.445
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hellström,KE
中科院分区：
文献类型：
--
作者：
Estin,CD;Stevenson,U;Kahn,M;Hellström,I;Hellström,KE
将人黑色素瘤相关抗原p97基因导入K1735小鼠黑色素瘤M2克隆的细胞中，当静脉注射入同基因C3H/HEN小鼠时，该细胞转移到肺。从转基因细胞中建立了肿瘤克隆，发现p97的表达水平不同。在免疫能力强的同基因小鼠中，它们的生长与p97抗原的表达呈负相关，当将表达较高水平的p97的品系注射到p97免疫的小鼠的足垫中时，会引发更强的迟发性超敏反应。5个高水平表达p97的克隆在免疫活性C3H/HEN小鼠中未能生长，而在裸鼠(nu/nu)中形成肿瘤。在体外培养时，表达水平最高的克隆2A的生长速度略快于其他任何一个克隆。由于几个转基因克隆被发现表达稳定水平的p97并具有一致的体内生长行为，它们为各种形式的抗原特异性主动和被动免疫治疗提供了有用的模型，这些药物与人类应用的药物相同。[美国国家癌症杂志81：445-448,1989]
The gene for the human melanoma-associated antigen p97 has been introduced by cDNA transfection into cells from clone M2 of the K1735 mouse melanoma, which metastasizes to the lung when injected iv into syngeneic C3H/HeN mice. Tumor clones were established from the transfected cells and found to differ in the level of p97 expression. Their outgrowth in immuno-competent syngeneic mice was shown to inversely correlate with p97 antigen expression, and lines that express higher p97 levels elicited a stronger delayed-type hypersensitivity response when injected into the footpads of mice immune to p97. Five clones which expressed very high levels of p97 failed to grow in immunocompetent C3H/HeN mice, while they formed tumors in nude (nu/nu) mice. The highest expressing clone, 2A, grew slightly faster than any of the other clones when cultured in vitro. Since several of the transfected clones were found to express a stable level of p97 and have consistent in vivo growth behavior, they provide a useful model for various forms of antigen-specific active and passive immunotherapy with the same agents as those intended for human application. [J Natl Cancer Inst 81:445–448, 1989]