Dynamic, Polymer-Integrated Crystals for Efficient, Reversible Protein Encapsulation
Dynamic, Polymer-Integrated Crystals for Efficient, Reversible Protein Encapsulation
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DOI：
10.1021/jacs.2c02584
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发表时间：
2022-06-15
影响因子：
15
通讯作者：
Tezcan, F. Akif
中科院分区：
文献类型：
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作者：
Han, Kenneth;Na, Youjeong;Tezcan, F. Akif
晶体材料越来越多地被用作封装蛋白质的平台，以创造稳定的功能材料。然而，晶体框架的吸收效率和刺激响应性受到其刚性的限制。我们最近报道了一种新形式的材料，聚合物集成晶体（PIX），它将蛋白质晶体的结构顺序与合成聚合物的动态、刺激响应特性结合起来。在这里，我们展示了 PIX 的结晶度、灵活性和化学可调性可用于以高装载效率（高达 46% w/w）封装客体蛋白。静电主客体相互作用能够实现客体蛋白的可逆、pH控制的摄取/释放以及主客体的相互稳定，从而为功能性生物材料的开发和生物大分子的受控递送创造了一个独特的协同平台。
Crystalline materials are increasingly being used as platforms for encapsulating proteins to create stable, functional materials. However, the uptake efficiencies and stimuli-responsiveness of crystalline frameworks are limited by their rigidities. We have recently reported a new form of materials, polymer-integrated crystals (PIX), which combine the structural order of protein crystals with the dynamic, stimuli-responsive properties of synthetic polymers. Here we show that the crystallinity, flexibility, and chemical tunability of PIX can be exploited to encapsulate guest proteins with high loading efficiencies (up to 46% w/w). The electrostatic host-guest interactions enable reversible, pH-controlled uptake/release of guest proteins as well as the mutual stabilization of the host and the guest, thus creating a uniquely synergistic platform toward the development of functional biomaterials and the controlled delivery of biological macromolecules.