Single-mismatch detection using gold-quenched fluorescent oligonucleotides
Single-mismatch detection using gold-quenched fluorescent oligonucleotides
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DOI：
10.1038/86762
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发表时间：
2001-04-01
影响因子：
46.9
通讯作者：
Libchaber, AJ
中科院分区：
文献类型：
--
作者：
Dubertret, B;Calame, M;Libchaber, AJ
在这里，我们描述了一种混合材料组成的单链DNA（ssDNA）分子，1.4 nm直径的金纳米粒子，和荧光团，是高度淬灭的纳米粒子通过距离依赖性的过程。当这种杂交分子与互补的ssDNA结合时，它的荧光会增加几千倍。我们表明，这种复合分子是一种不同类型的分子信标，灵敏度提高了100倍。在竞争性杂交试验中，用这种探针检测单错配的能力是用其他分子信标的8倍。
Here we describe a hybrid material composed of a single-stranded DNA (ssDNA) molecule, a 1.4 nm diameter gold nanoparticle, and a fluorophore that is highly quenched by the nanoparticle through a distance-dependent process. The fluorescence of this hybrid molecule increases by a factor of as much as several thousand as it binds to a complementary ssDNA. We show that this composite molecule is a different type of molecular beacon with a sensitivity enhanced up to 100-fold. In competitive hybridization assays, the ability to detect singlemismatch is eightfold greater with this probe than with other molecular beacons.