Self-replicative RNA vaccines elicit protection against influenza A virus, respiratory syncytial virus, and a tickborne encephalitis virus
Self-replicative RNA vaccines elicit protection against influenza A virus, respiratory syncytial virus, and a tickborne encephalitis virus
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DOI：
10.1086/319857
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发表时间：
2001-05-01
影响因子：
6.4
通讯作者：
Liljeström, P
中科院分区：
文献类型：
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作者：
Fleeton, MN;Chen, M;Liljeström, P
在基因疫苗接种中，接受者用编码抗原的核酸（通常是DNA）进行免疫。本研究探讨了使用重组甲病毒RNA分子作为基因疫苗接种新方法的可能性，该分子在转染细胞的细胞质中复制。用编码来自以下3种病毒之一的包膜蛋白的重组塞姆利基森林病毒RNA对小鼠进行免疫：甲型流感病毒、一种蜱传黄病毒（跳跃病病毒）或呼吸道合胞病毒（RSV）。血清学分析表明引发了抗原特异性抗体反应。IgG分型显示，用编码RSV F蛋白的RNA免疫后主要诱导了Th1型免疫反应，这与预防RSV感染相关。攻毒感染表明，RNA免疫引发了对3种模型病毒性疾病的显著保护水平。
In genetic vaccination, recipients are immunized with antigen-encoding nucleic acid, usually DNA. This study addressed the possibility of using the recombinant alpha virus RNA molecule, which replicates in the cytoplasm of transfected cells, as a novel approach for genetic vaccination. Mice were immunized with recombinant Semliki Forest virus RNA-encoding envelope proteins from one of 3 viruses: influenza A virus, a tickborne flavivirus (louping ill virus), or respiratory syncytial virus (RSV). Serologic analyses showed that antigen-specific antibody responses were elicited. IgG isotyping indicated that predominantly Th1 type immune responses were induced after immunization with RSV F protein-encoding RNA, which is relevant for protection against RSV infection. Challenge infection showed that RNA immunization had elicited significant levels of protection against the 3 model virus diseases.