Molecular basis off hurthle cell papillary thyroid carcinoma.
Molecular basis off hurthle cell papillary thyroid carcinoma.
复制标题
甲状腺乳头状细胞癌的分子基础。
DOI：
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发表时间：
2000
影响因子：
5.8
通讯作者：
S. Asa
中科院分区：
文献类型：
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作者：
C. Cheung;S. Ezzat;L. Ramyar;J. Freeman;S. Asa
在甲状腺肿瘤中，Hurthle细胞瘤（HCT）传统上是一个独特的诊断类别。Hurthle细胞腺瘤是良性的包裹性滤泡病变。Hurthle细胞癌表现出明确的包膜和/或血管浸润;它们是预后不良的侵袭性肿瘤。最近，Hurthle细胞乳头状甲状腺癌（PTC）已确定的形态学基础。我们假设，一个子集的HCT代表PTC的临床，组织学和免疫组化特征的基础上特定的分子事件。ret/PTC基因重排产生PTC特有的新癌基因。我们研究了一组（n = 50）的HCT的ret/PTC基因重排。通过光镜评价细胞核特征、RT-PCR检测ret/PTC基因重排和免疫组化检测ret，检查肿瘤的乳头状分化。在24例非侵袭性肿瘤中，13例含有ret/PTC-1、-2或-3的核糖核酸，其中9例为ret免疫反应阳性。在19例Hurthle细胞癌中，15例有局灶性核染色质减退伴沟槽和/或夹杂物;表达ret/PTC-1、-2或-3转录本;并显示ret阳性。ret/PTC基因重排的肿瘤倾向于淋巴结转移而不是血行扩散。我们的研究结果表明，一个子集的HCT表现出PTC的功能，可归因于特定的基因重排，导致ret/PTC癌基因的表达。这些数据支持将HCT细分为三组：Hurthle细胞腺瘤、Hurthle细胞癌和Hurthle细胞PTC。
Among thyroid neoplasms, Hurthle cell tumors (HCTs) have traditionally been a distinct diagnostic category. Hurthle cell adenomas are encapsulated follicular lesions with benign behavior. Hurthle cell carcinomas exhibit unequivocal capsular and/or vascular invasion; they are aggressive tumors with a poor prognosis. Recently, Hurthle cell papillary thyroid carcinomas (PTCs) have been identified on morphological grounds. We hypothesize that a subset of HCTs represent PTC with clinical, histological, and immunohistochemical features based on specific molecular events. ret/PTC gene rearrangements give rise to novel oncogenes that are unique to PTC. We studied a group (n = 50) of HCTs for ret/PTC gene rearrangements. Tumors were examined for papillary differentiation by light microscopic evaluation of nuclear features, by RT-PCR for ret/PTC gene rearrangements, and by immunohistochemistry for ret. Among 24 noninvasive tumors, 13 contained ribonucleic acid for ret/PTC-1, -2, or -3, and 9 of these were immunoreactive for ret. Among 19 Hurthle cell carcinomas, 15 had focal nuclear hypochromasia with grooves and/or inclusions; expressed transcripts of ret/PTC-1, -2, or-3; and exhibited ret positivity. Tumors with ret/PTC gene rearrangements tended to have lymph node metastases rather than hematogenous spread. Our results indicate that a subset of HCTs exhibit features of PTC that are attributable to specific gene rearrangements, resulting in expression of ret/PTC oncogenes. These data support subclassification of HCTs into three groups: Hurthle cell adenomas, Hurthle cell carcinomas, and Hurthle cell PTC.