Genetic, Genomic, and Imaging Approaches to Dissect the Role of Polycomb Group Epigenetic Regulators in Mice
Genetic, Genomic, and Imaging Approaches to Dissect the Role of Polycomb Group Epigenetic Regulators in Mice
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遗传、基因组和成像方法剖析多梳组表观遗传调节因子在小鼠中的作用
DOI：
10.1007/978-1-0716-2481-4_10
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发表时间：
2022
影响因子：
--
通讯作者：
Koseki Haruhiko
中科院分区：
文献类型：
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作者：
Yakushiji-Kaminatsui Nayuta;Kondo Takashi;Ohinata Yasuhide;Takano Junichiro;Koseki Haruhiko
在后生动物发育中最重要的组蛋白甲基转移酶是EZH 1/2及其同源物，其甲基化组蛋白H3赖氨酸27，并作为一组高度保守的染色质调节因子的一部分，称为Polycomb Group（PcG）蛋白。要精确了解PcG蛋白在协调分化和维持细胞身份中的作用，需要多种遗传和分子方法。在这里，我们提出了一套完整的方法研究PcG蛋白在早期小鼠发育，包括突变株的产生，胚胎干细胞的衍生，表观基因组分析，免疫荧光和原位杂交。
Among the most important histone methyltransferases for metazoan development are EZH1/2 and their homologs, which methylate histone H3 lysine 27 and act as part of a highly conserved set of chromatin regulators called Polycomb Group (PcG) proteins. Reaching a precise understanding of the roles of PcG proteins in the orchestration of differentiation and the maintenance of cell identity requires a variety of genetic and molecular approaches. Here, we present a full suite of methods for the study of PcG proteins in early murine development, including mutant strain generation, embryonic stem cell derivation, epigenomic profiling, and immunofluorescence andin situhybridization.