A Prunus mume Extract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells
A Prunus mume Extract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells
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DOI：
10.1271/bbb.110264
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发表时间：
2011-10-01
影响因子：
1.6
通讯作者：
Utsunomiya, Hirotoshi
中科院分区：
文献类型：
--
作者：
Kono, Ryohei;Okuno, Yoshiharu;Utsunomiya, Hirotoshi
骨质疏松症是一种严重的骨质疏松症。骨中存在恒定的基质重塑，其中骨形成由成骨细胞进行，而骨吸收由破骨细胞完成。我们研究了日本杏（梅SIBE。et ZUCC.）提取物对前成骨细胞MC 3 T3-E1细胞增殖和成骨分化的影响。采用碱性磷酸酶（ALP）活性测定、细胞增殖试验、茜素红染色及成骨细胞基因表达分析等方法，观察成骨细胞的增殖和分化情况。梅花水溶性组分（PWF）可提高ALP活性，促进细胞增殖和矿化。骨桥蛋白和骨形态发生蛋白-2的基因表达，这是在成骨细胞分化的早期阶段的标志物，被PWF处理显着增强。因此，PWF刺激细胞增殖和成骨细胞分化，并可能具有预防骨质疏松症的潜力。
Osteoporosis is a serious disease caused by decreased bone mass. There is constant matrix remodeling in bones, by which bone formation is performed by osteoblastic cells, whereas bone resorption is accomplished by osteoclast cells. We investigated the effect of a Japanese apricot (Prunus mume SIBE. et ZUCC.) extract on the proliferation and osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. An alkaline phosphatase (ALP) activity assay, cell proliferation assay, alizarin red staining and expression analysis of osteoblastic genes were carried out to assess the proliferation and osteoblastic differentiation. The water-soluble fraction of Prunus mume (PWF) increased the ALP activity, cell proliferation and mineralization. The gene expression of osteopontin and bone morphogenetic protein-2, which are markers in the early period of osteoblastic differentiation, were significantly enhanced by the PWF treatment. PWF therefore stimulated the proliferation and osteoblastic differentiation of cells and may have potential to prevent osteoporosis.