Role of the nitric oxide-cyclic GMP pathway in regulation of vaginal blood flow
Role of the nitric oxide-cyclic GMP pathway in regulation of vaginal blood flow
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DOI：
10.1038/sj.ijir.3901038
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发表时间：
2003-10-01
影响因子：
2.6
通讯作者：
Traish, AM
中科院分区：
文献类型：
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作者：
Kim, SW;Jeong, SJ;Traish, AM
一氧化氮（NO）在阴道灌注中的调节作用仍不清楚。我们使用了 NO-环鸟苷酸 (NO-cGMP) 途径中酶的特异性抑制剂，并研究了它们对兔子阴道血流的影响。兔阴道近端和远端的一氧化氮合酶（NOS）活性相似；而远端阴道的精氨酸酶活性高出 3.4 倍。静脉注射 NOS 抑制剂 L-NAME 导致生殖器组织氧合血红蛋白减少 66%，阴道血流量减少 53%。尽管全身动脉压增加了 20-30%，但这种衰减还是发生了。精氨酸酶抑制剂 ABH 导致生殖器组织氧合血红蛋白增加 2.1 倍，阴道血流量增加 34%。鸟苷酸环化酶抑制剂 1H-[1,2,4]恶二唑并[4,3,-a]喹喔啉-1-一和磷酸二酯酶 5 型抑制剂西地那非分别导致阴道血流量减少 37% 和增加 44%。这些观察结果表明，NO-cGMP 途径是阴道血流动力学的重要调节因子。
The regulatory role of nitric oxide (NO) in vaginal perfusion remains unclear. We used specific inhibitors of enzymes in the NO-cyclic GMP (NO-cGMP) pathway and investigated their effects on vaginal blood flow in the rabbit. NO synthase (NOS) activity was similar in both the proximal and distal rabbit vagina; whereas, arginase activity was 3.4-fold higher in the distal vagina. Intravenous administration of the NOS inhibitor L-NAME resulted in a 66% reduction in genital tissue oxyhemoglobin and a 53% reduction in vaginal blood flow. This attenuation occurred despite a 20-30% increase in systemic arterial pressure. The arginase inhibitor ABH caused a 2.1-fold increase in genital tissue oxyhemoglobin and 34% increase in vaginal blood flow. The guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one and the phosphodiesterase type 5 inhibitor sildenafil caused in a 37% reduction and a 44% increase in vaginal blood flow, respectively. These observations suggest that the NO-cGMP pathway is an important regulator of vaginal hemodynamics.