Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential
Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential
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DOI：
10.5114/aoms.2018.73128
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发表时间：
2019-05-01
影响因子：
3.8
通讯作者：
Feng, Jun-wei
中科院分区：
文献类型：
--
作者：
Long, Hou-yong;Huang, Qing-xian;Feng, Jun-wei
简介：本研究旨在观察去氢木香内酯对人骨肉瘤细胞株BON-1的增殖抑制作用，并探讨其可能的作用机制。材料与方法：采用MTT法检测去氢木香内酯对BON-1细胞的细胞毒作用。荧光和透射电子显微镜（TEM）技术被用来研究该化合物对细胞形态和凋亡的影响。流式细胞术用于评估对细胞周期时相分布的影响。结果：去氢木香内酯对人骨肉瘤BON-1细胞的生长有明显的抑制作用（P < 0.01），对人骨肉瘤BON-1细胞的增殖有明显的抑制作用（P < 0.01）。去氢木香内酯对BON-1的生长抑制作用具有时间和浓度依赖性。发现脱氢木香内酯在24和48小时时间间隔的IC 50分别为71.9 μ M和52.3 μ M。脱氢木香内酯的生长抑制作用被认为是由于线粒体膜电位的损失，诱导细胞凋亡和亚G1细胞周期arrest.Conclusions：脱氢木香抑制体外胃泌素瘤癌细胞的生长，因此可能被证明是有益的胃泌素瘤癌的管理。
Introduction: The purpose of the present study was to evaluate the antipro-liferative activity of dehydrocostus lactone against human BON-1 cancer cell lines and to explore the possible underlying mechanism.Material and methods: MTT cell viability assay was used to determine cytotoxic effects of dehydrocostus lactone in BON-1 cells. Fluorescence and transmission electron microscopic (TEM) techniques were used to study the effect of the compound on cellular morphology and apoptosis. Flow cytometry was used to assess the effect on cell cycle phase distribution. Effects of the drug on cell apoptosis and mitochondrial membrane potential were analyzed by flow cytometry using annexin v and rhodamine-123 as fluorescent probes.Results: The results of the present study indicated that dehydrocostus lactone significantly (p < 0.01) inhibited the growth of BON-1 cancer cells. These growth inhibitory effects of dehydrocostus lactone on BON-1 were found to be time and concentration-dependent. The IC50 of dehydrocostus lactone were found to be 71.9 mu M and 52.3 mu M at 24 and 48 h time intervals respectively. The growth inhibitory effects of dehydrocostus lactone were found to be due to loss of mitochondrial membrane potential, the induction of apoptosis and sub-G1 cell cycle arrest.Conclusions: Dehydrocostus inhibits in vitro gastrinoma cancer cell growth and therefore may prove beneficial in the management of gastrinoma cancer.