Characterisation of uniformly 13C, 15N labelled bacteriochlorophyll a and bacteriopheophytin a in solution and in solid state: complete assignment of the 13C, 1H and 15N chemical shifts
Characterisation of uniformly 13C, 15N labelled bacteriochlorophyll a and bacteriopheophytin a in solution and in solid state: complete assignment of the 13C, 1H and 15N chemical shifts
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溶液和固态中均匀 13C、15N 标记的细菌叶绿素 a 和细菌脱镁叶绿素 a 的表征：13C、1H 和 15N 化学位移的完整分配
DOI：
10.1002/mrc.2295
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发表时间：
2008
影响因子：
2
通讯作者：
H. D. de Groot
中科院分区：
文献类型：
--
作者：
T. Egorova;B. van Rossum;C. Erkelens;H. D. de Groot
本文报道了两种细菌光合色素细菌叶绿素a（BChl）和细菌脱镁叶绿素a（BPheo）的~（13）C、~ 1H和~（15）N的全谱归属和固态化学位移。开发了统一的稳定同位素标记策略，并将其应用于光合色素的生物合成制备，即均匀13 C，15 N标记的BChl a和BPheo a。用13 C、15 N进行均匀稳定同位素标记允许进行13 C、15 N和1H共振的分配。从生长在均匀富集13 C（99%）和15 N（98%）的培养基中的光合细菌Rhodoptera palustris 17001的生物量中分离光合色素。两种颜料均通过溶液（丙酮-d 6）中的NMR和固态中的MAS NMR表征，并通过标准液体2D 13 C 13 C COSY、1H 13 C HMQC、1H 15 N HMBC和固体2D 13 C 13 C RFDR、1H 13 C FSLG HETCOR和1H 15 N HETCOR相关技术在600 MHz和750 MHz下记录和归属它们的NMR共振。色素的表征是从生物化学到制药工业、光合作用和食品研究的兴趣所在。版权所有© 2008约翰威利父子有限公司。
In this investigation we report a complete assignment of 13C, 1H and 15N solution and solid state chemical shifts of two bacterial photosynthetic pigments, bacteriochlorophyll (BChl) a and bacteriopheophytin (BPheo) a. Uniform stable‐isotope labelling strategies were developed and applied to biosynthetic preparation of photosynthetic pigments, namely uniformly 13C, 15N labelled BChl a and BPheo a. Uniform stable‐isotope labelling with 13C, 15N allowed performing the assignment of the 13C, 15N and 1H resonances. The photosynthetic pigments were isolated from the biomass of photosynthetic bacteria Rhodopseudomonas palustris 17001 grown in uniformly 13C (99%) and 15N (98%) enriched medium. Both pigments were characterised by NMR in solution (acetone‐d6) and by MAS NMR in solid state and their NMR resonances were recorded and assigned through standard liquid 2D 13C13C COSY, 1H13C HMQC, 1H15N HMBC and solid 2D 13C13C RFDR, 1H13C FSLG HETCOR and 1H15N HETCOR correlation techniques at 600 MHz and 750 MHz. The characterisation of pigments is of interest from biochemical to pharmaceutical industries, photosynthesis and food research. Copyright © 2008 John Wiley & Sons, Ltd.