FINE-STRUCTURAL LOCALIZATION OF ADENOSINE-TRIPHOSPHATASE ACTIVITY ON TASTE BUD IN FUNGIFORM PAPILLAE OF RAT
FINE-STRUCTURAL LOCALIZATION OF ADENOSINE-TRIPHOSPHATASE ACTIVITY ON TASTE BUD IN FUNGIFORM PAPILLAE OF RAT
复制标题
DOI：
10.1679/aohc1950.40.63
复制
发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
ODA, M
中科院分区：
文献类型：
--
作者：
AKISAKA, T;ODA, M
用电镜组织化学方法观察了Mg ~（2+）激活的ATP酶活性在大鼠菌状味蕾中的分布。在味觉细胞的细胞膜上发现了反应产物，但不在它们的细胞质内。味觉孔中发生了特别强烈的反应。此外，在轴突-雪旺细胞和轴突-I型细胞间隙中有酶的表达，而在神经纤维和II型细胞的接触处无酶的表达。通过反应产物的存在，可以将味蕾与周围的上皮区分开来。ATP酶活性是不存在的突触部分III型细胞面临的神经纤维。沿基底层沿着未见反应产物。在菌状芽中，神经纤维经常相互接触，这些部位缺乏ATP酶的沉积。
The localization of Mg2+ activated ATPase activity in rat fungiform taste buds was demonstrated by EM histochemistry. The reaction product was found on the cell membrane of the taste cells, but not within their cytoplasm. An especially strong reaction occurred in the taste pore. Besides, enzymes were observed in axon-Schwann cell and axon-type I cell interspace, but not in the contacts of the nerve fiber and type II cell. The taste bud could be distinguished from the surrounding epithelium by the existence of the reaction product. ATPase activity was absent at the synaptic portion where type III cells faced nerve fibers. No reaction product was seen along the basal lamina. In fungiform buds, the nerve fibers were frequently shown to make contact with each other, and these sites were devoid of deposits for ATPase.