Solvatochromic Near-Infrared Probe for Polarity Mapping of Biomembranes and Lipid Droplets in Cells under Stress
Solvatochromic Near-Infrared Probe for Polarity Mapping of Biomembranes and Lipid Droplets in Cells under Stress
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DOI：
10.1021/acs.jpclett.9b00668
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发表时间：
2019-05-16
影响因子：
5.7
通讯作者：
Klymchenko, Andrey S.
中科院分区：
文献类型：
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作者：
Ashoka, Anila Hoskere;Ashokkumar, Pichandi;Klymchenko, Andrey S.
极性敏感荧光染料能否监测活细胞对基本应激条件的反应，如营养缺乏和氧化应激？为了解决这个问题，我们开发了一种推挽式二氧硼探针（DXB-NIR），用于生物膜和脂滴，其在远红外到近红外区域具有强的溶剂化显色性，高荧光亮度，光稳定性和双光子吸收截面，在930 nm处达到13800 GM。在模型膜中，DXB-NIR在液体有序和无序膜相之间表现出前所未有的80 nm的位移，允许分离膜微区的稳健成像。用DXB-NIR对活细胞进行双色成像可以在质膜、内质网和脂滴中进行极性映射，这表明饥饿和氧化应激会增加局部极性，并且这种变化对于每个研究的细胞室都是不同的。因此，通过推动现有的溶剂化显色染料的限制，我们引入了极性映射的概念，用于监测细胞对压力的反应。
Can polarity-sensitive fluorescent dyes monitor the response of live cells to fundamental stress conditions, such as deprivation from nutrition and oxidative stress? To address this question, we developed a push-pull dioxaborine probe (DXB-NIR) for biomembranes and lipid droplets featuring strong solvatochromism in the far-red to near-infrared region, high fluorescence brightness, photostability, and two-photon absorption cross section, reaching 13800 GM at 930 nm. In model membranes, DXB-NIR exhibits unprecedented 80 nm shift between liquid ordered and disordered membrane phases, allowing robust imaging of separated membrane microdomains. Two-color imaging of live cells with DXB-NIR enables polarity mapping in plasma membranes, endoplasmic reticulum, and lipid droplets, which reveals that starvation and oxidative stress produce an increase in the local polarity, and this change is different for each of the studied cell compartments. Thus, by pushing the limits of existing solvatochromic dyes, we introduce a concept of polarity mapping for monitoring the response of cells to stress.