3-DIMENSIONAL RECONSTRUCTION OF CELLS FROM SERIAL SECTIONS AND WHOLE-CELL MOUNTS USING MULTILEVEL CONTOURING OF STEREO MICROGRAPHS
3-DIMENSIONAL RECONSTRUCTION OF CELLS FROM SERIAL SECTIONS AND WHOLE-CELL MOUNTS USING MULTILEVEL CONTOURING OF STEREO MICROGRAPHS
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DOI：
10.1002/jemt.1060090406
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发表时间：
1988-08-01
期刊：
影响因子：
--
通讯作者：
PARSONS, D
中科院分区：
文献类型：
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作者：
MARKO, M;LEITH, A;PARSONS, D
描述了一个全面的基于计算机图形学的系统（STERECON），用于跟踪和数字化单个或立体对电子显微照片的轮廓。在显微照片内的平行平面中绘制轮廓。还提供了在沿沿着线性结构（例如细胞骨架元件）的任何方向上的全三维（3-D）坐标中的追踪和数字化。立体对显微照片与在图形终端监视器上追踪的轮廓结合起来观察。这可以通过将原始电子显微镜（EM）底片投影到屏幕上并将这些图像与在监视器上绘制的轮廓线光学组合来完成，或者通过首先将图像数字化并将它们与轮廓线一起沿着直接显示在监视器上来完成。先前的图像数字化允许计算机增强待轮廓化的结构。校正和对齐例程包括处理可变的切片厚度，部分失真和质量损失，在电子显微镜摄影的变化，和终端屏幕曲率时，结合投影图像与监视器上的轮廓线。STERECON系统将连续切片的数字化数据组织并显示为三维重建。重建可以在任何方向上作为删除隐藏线的轮廓堆栈、线框模型或具有可变照明、透明度和反射率的着色实体模型进行查看。可以确定重建对象的面积和表面积。特别注意使系统方便生物用户。用户可以选择三种不同的立体观看方法。
A comprehensive computer‐graphics‐based system (STERECON) is described for tracing and digitizing contours from individual or stereopair electron micrographs. The contours are drawn in parallel planes within the micrographs. Provision is also made for tracing and digitizing in full three‐dimensional (3‐D) coordinates in any direction along linear structures such as cytoskeletal elements. The stereopair micrographs are viewed in combination with the contours being traced on a graphics terminal monitor. This is done either by projecting original electron micrograph (EM) negatives onto a screen and optically combining these images with contour lines being drawn on the monitor, or by first digitizing the images and displaying them directly on the monitor along with the contour lines. Prior image digitization allows computer enhancement of the structures to be contoured. Correction and alignment routines are included to deal with variable section thickness, section distortion and mass loss, variations in photography in the electron microscope, and terminal screen curvature when combining projected images with contour lines on the monitor. The STERECON system organizes and displays the digitized data from successive sections as a 3‐D reconstruction. Reconstructions can be viewed in any orientation as contour stacks with hidden lines removed; as wire‐frame models; or as shaded, solid models with variable lighting, transparency, and reflectivity. Volumes and surface areas of the reconstructed objects can be determined. Particular attention was paid to making the system convenient for the biological user. Users are given a choice of three different stereo‐viewing methods.