Perilipin expression in human adipose tissues: effects of severe obesity, gender, and depot.
Perilipin expression in human adipose tissues: effects of severe obesity, gender, and depot.
复制标题
DOI：
10.1038/oby.2003.128
复制
发表时间：
2003-08
期刊：
影响因子：
--
通讯作者：
Yanxin Wang;Sean Sullivan;M. Trujillo;Mi-Jeong Lee;S. Schneider;R. Brolin;Y. Kang;Yaron Werber;A. Greenberg;S. Fried
中科院分区：
文献类型：
--
作者：
Yanxin Wang;Sean Sullivan;M. Trujillo;Mi-Jeong Lee;S. Schneider;R. Brolin;Y. Kang;Yaron Werber;A. Greenberg;S. Fried
目的：周脂质是一种磷蛋白，位于脂肪细胞内三酰甘油液滴的表面，调节脂肪分解速率。我们试图确定严重肥胖和储存[网膜 (Om) 与皮下 (Sc)] 对个体脂肪组织中周脂蛋白表达的影响。研究方法和程序：通过 Northern 和 Western 分析，对在手术中从严重肥胖受试者获得的 Om 和 Sc 脂肪组织样本以及来自非肥胖受试者的脂肪抽吸物进行周脂蛋白和 mRNA 水平的分析。结果：周脂蛋白 A (periA) 是在个体脂肪组织中表达的主要周脂蛋白。脂肪组织。与非肥胖受试者相比，重度肥胖受试者的 Sc 脂肪组织中 periA mRNA 相对丰度显着较低。脂肪组织提取物的蛋白质印迹显示，与非肥胖受试者相比，严重肥胖者腹部 Sc 脂肪组织中表达的 periA 蛋白水平相对于组织蛋白或脂肪细胞表面积显着较低（~ -40%）。然而，计算出的每个脂肪细胞的周脂质质量在两组之间没有差异。与肥胖个体的 Om 脂肪组织相比，Sc 中的 Perilipin mRNA 水平较高（p < 0.025；n = 26；17 名女性，9 名男性）；然而，periA 蛋白水平没有差异。此外，肥胖男性的 Sc 脂肪组织中周脂肪蛋白（而非 mRNA）水平高于女性 (p<0.025)。 讨论：周脂肪蛋白表达的变化可能导致肥胖个体与非肥胖个体以及肥胖女性与肥胖男性相比基础脂肪分解率较高。
Objective: Perilipins are phosphoproteins that are localized to the surface of triacylglycerol droplets within adipocytes where they regulate the rate of lipolysis. We sought to determine the effects of severe obesity and depot [omental (Om) vs. subcutaneous (Sc)] on perilipin expression in the adipose tissue of individuals.Research Methods and Procedures: Samples of Om and Sc adipose tissues obtained at surgery from severely obese subjects and fat aspirations from nonobese subjects were analyzed for perilipin protein and mRNA levels by Northern and Western analysis.Results: Perilipin A (periA) was the major perilipin expressed in adipose tissues. periA mRNA relative abundance was significantly lower in Sc adipose tissue from severely obese compared to that from nonobese subjects. Western blotting of adipose tissue extracts showed that periA protein levels expressed relative to tissue protein or fat cell surface area were significantly lower (∼ −40%) in abdominal Sc adipose tissue from severely obese compared to that from nonobese subjects. However, the calculated mass of perilipin per fat cell did not differ between the two groups. Perilipin mRNA levels were higher in Sc compared to Om adipose tissue from obese individuals (p< 0.025;n= 26; 17 women, 9 men); however, periA protein levels did not differ. In addition, perilipin protein, but not mRNA, levels were higher in Sc adipose tissue from obese men than from women (p< 0.025).Discussion: Variations in perilipin expression may contribute to the higher basal lipolytic rates observed in obese compared to nonobese individuals and in obese women compared to obese men.