Effect of Fluorinert on the Histological Properties of Formalin-Fixed Human Brain Tissue
Effect of Fluorinert on the Histological Properties of Formalin-Fixed Human Brain Tissue
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DOI：
10.1093/jnen/nly098
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发表时间：
2018-12-01
影响因子：
3.2
通讯作者：
Holton, Janice L.
中科院分区：
文献类型：
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作者：
Iglesias, Juan Eugenio;Crampsie, Shauna;Holton, Janice L.
氟化（全氟化碳）代表了在离体脑MRI扫描中最小化易感性伪影的廉价选择，并提供了一种替代福林林的选择。然而，其对固定组织和组织学分析的影响尚未得到严格和定量的验证。在本研究中，我们从5例福尔马林固定标本（2例进行性核上性麻痹病例，3例对照组）的两个脑区（额极和小脑）切除组织块。每个病例每个区域切除2块（共20块），其中一块随后在氟化剂中浸泡一周，然后放回含有福尔马林的容器中。每个区域的其他块保存在福尔马林中作为对照。然后对组织块进行切片，并对每个组织块进行组织学分析，包括常规染色和免疫组织化学。由经验丰富的神经病理学家通过显微镜对染色的组织学切片进行目视检查，发现任何样品之间没有任何明显的差异。此外，基于自动图像补丁分类的定量分析表明，基于深度卷积神经网络的最先进分类器几乎无法区分样本。结果表明，即使经过长时间（1周）的浸泡，Fluorinert对随后的组织组织学分析也没有影响，这对于最长的扫描方案来说也是足够的。
Fluorinert (perfluorocarbon) represents an inexpensive option for minimizing susceptibility artifacts in ex vivo brain MRI scanning, and provides an alternative to Fomblin. However, its impact on fixed tissue and histological analysis has not been rigorously and quantitatively validated. In this study, we excised tissue blocks from 2 brain regions (frontal pole and cerebellum) of 5 formalin-fixed specimens (2 progressive supranuclear palsy cases, 3 controls). We excised 2 blocks per region per case (20 blocks in total), one of which was subsequently immersed in Fluorinert for a week and then returned to a container with formalin. The other block from each region was kept in formalin for use as control. The tissue blocks were then sectioned and histological analysis was performed on each, including routine stains and immunohistochemistry. Visual inspection of the stained histological sections by an experienced neuropathologist through the microscope did not reveal any discernible differences between any of the samples. Moreover, quantitative analysis based on automated image patch classification showed that the samples were almost indistinguishable for a state-of-the-art classifier based on a deep convolutional neural network. The results showed that Fluorinert has no effect on subsequent histological analysis of the tissue even after a long (1 week) period of immersion, which is sufficient for even the lengthiest scanning protocols.