The tegument protein UL94 of human cytomegalovirus as a binding partner for tegument protein pp28 identified by intracellular imaging
The tegument protein UL94 of human cytomegalovirus as a binding partner for tegument protein pp28 identified by intracellular imaging
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通过细胞内成像鉴定人巨细胞病毒的外皮蛋白 UL94 作为外皮蛋白 pp28 的结合伴侣。
DOI：
10.1016/j.virol.2009.03.007
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发表时间：
2009-05-25
期刊：
影响因子：
3.7
通讯作者：
Zhang, Xian-En
中科院分区：
文献类型：
--
作者：
Liu, Yalan;Cui, Zongqiang;Zhang, Xian-En
人巨细胞病毒（human cytomegalovirus，HCMV）被膜蛋白pp 28是HCMV感染性病毒粒子组装所必需的蛋白质，但其在HCMV被膜和增殖过程中的功能尚不清楚。通过使用活细胞荧光共振能量转移（FRET）显微镜和酵母双杂交试验，我们发现，另一个HCMV被膜蛋白，UL 94，是一个特异性的结合伴侣的pp 28。pp 28和UL 94之间的相互作用在一个点状的、非核区室中成像，该区室先前被指定为病毒组装区室（AC）。pp 28的氨基酸22-43被鉴定为负责其与UL 94的结合，而在UL 94内未发现线性结合位点。pp 28和UL 94之间的相互作用可能是HCMV衣壳化、盖层化和包埋过程中的一个环节。本研究为进一步研究HCMV被膜蛋白在HCMV病毒粒子组装中的作用奠定了基础。(C)2009爱思唯尔公司All rights reserved.
The tegument protein pp28 of human cytomegalovirus (HCMV) is essential for the assembly of infectious HCMV virions, but how it functions during the process of HCMV tegumentation and envelopment remains unclear. By using live cell fluorescence resonance energy transfer (FRET) microscopy and yeast two-hybrid assays, we found that another HCMV tegument protein, UL94, was a specific binding partner for pp28. The interaction between pp28 and UL94 was imaged in a punctuate, juxtanuclear compartment, previously designated as the virus assembly compartment (AC). Amino acids 22-43 of pp28 were identified as being responsible for its binding with UL94, while no linear binding site Could be found within UL94. The interaction between pp28 and UL94 may serve as a link in the sequential processes of HCMV capsidation, tegumentation and envelopment. This Study provides a foundation for further Studies into how the HCMV tegument proteins act in the assembly of HCMV virions. (C) 2009 Elsevier Inc. All rights reserved.