Human cytomegalovirus UL84 protein contains two nuclear export signals and shuttles between the nucleus and the cytoplasm
Human cytomegalovirus UL84 protein contains two nuclear export signals and shuttles between the nucleus and the cytoplasm
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DOI：
10.1128/jvi.00995-06
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发表时间：
2006-10-01
影响因子：
5.4
通讯作者：
Stamminger, Thomas
中科院分区：
文献类型：
--
作者：
Lischka, Peter;Rauh, Claudia;Stamminger, Thomas
以前的研究将人巨细胞病毒pUL 84定义为具有核定位的必需调节蛋白，其被提出在病毒DNA合成的起始期间起作用。最近，我们证明了pUL 84内的282个氨基酸的复杂结构域作为非常规的核定位信号。该结构域的序列检查揭示了与富含亮氨酸的核输出信号同源的基序的存在。在这里，我们报告的两个功能，自主核出口信号的识别，并显示，pUL 84作为CRM-1依赖的核质穿梭蛋白。这表明这种必需的病毒调节蛋白具有意想不到的细胞质作用。
Previous studies defined pUL84 of human cytomegalovirus as an essential regulatory protein with nuclear localization that was proposed to act during initiation of viral-DNA synthesis. Recently, we demonstrated that a complex domain of 282 amino acids within pUL84 functions as a nonconventional nuclear localization signal. Sequence inspection of this domain revealed the presence of motifs with homology to leucine-rich nuclear export signals. Here, we report the identification of two functional, autonomous nuclear export signals and show that pUL84 acts as a CRM-1-dependent nucleocytoplasmic shuttling protein. This suggests an unexpected cytoplasmic role for this essential viral regulatory protein.