Complete amino acid sequence of human brain calmodulin.
Complete amino acid sequence of human brain calmodulin.
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DOI：
10.1021/bi00539a041
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发表时间：
1982-05
期刊：
影响因子：
2.9
通讯作者：
T. Sasagawa;L. Ericsson;K. Walsh;W. E. Schreiber;E. Fischer;K. Titani
中科院分区：
文献类型：
--
作者：
T. Sasagawa;L. Ericsson;K. Walsh;W. E. Schreiber;E. Fischer;K. Titani
用胰蛋白酶消化法测定了人脑钙调素的氨基酸全序列。金黄色葡萄球菌V8蛋白酶和溴化氰。通过反相高效液相色谱法纯化肽，并用测序仪进行分析。该蛋白含有148个氨基酸残基，分子量为16792。与其他钙调素一样，蛋白质的氨基末端残基被乙酰基封闭，三甲基赖氨酸残基位于115位。该序列与其他物种中完全确定的序列之间的唯一差异是酰胺基团的分配。
The complete amino acid sequence of calmodulin from human brain has been determined by using peptides derived from digests with trypsin. Staphylococcus aureus V8 protease, and cyanogen bromide. The peptides were purified by means of reversed-phase high-performance liquid chromatography and analyzed with a sequenator. The protein contains 148 amino acid residues and has a molecular weight of 16792. As in other calmodulins, the amino-terminal residue of the protein is blocked by an acetyl group, and a trimethyllysine residue is located at position 115. The only difference between this sequence and those fully determined in other species is the assignment of amide groups.