Short tandem target mimics inhibit Chlamydomonas reinhardtii microRNAs
Short tandem target mimics inhibit Chlamydomonas reinhardtii microRNAs
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短串联靶模拟物抑制莱茵衣藻 microRNA
DOI：
10.1016/j.algal.2020.101824
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发表时间：
2020-05
期刊：
影响因子：
--
通讯作者：
Zhangli Hu
中科院分区：
文献类型：
--
作者：
Ting Sun;Yuting Wang;Muhammad Anwar;Sulin Lou;Yanfeng Zeng;Hui Li;Zhangli Hu
短串联靶向模拟(STTM)RNA含有两个由间隔区连接的不可切割的microRNA(MiRNA)结合位点，可用于有效地阻断动植物中特定miRNAs的功能。然而，STTMS在单细胞生物体中的应用尚未见报道。在本研究中，我们测试了不同的针对莱茵衣藻miRNAs的STTM，以验证这种方法在单细胞生物体中的可行性。以已鉴定的3个致病基因(miR906-5P、miR1166.1和miR1150.3)为靶标，构建了含有STTM的转基因藻类。与非转基因对照相比，所有三个STTM构建物诱导的miRNA水平都显著降低，miR1150.3的水平降低了84%，miR1166.1的水平降低了73%。令人惊讶的是，这种对miRNA水平的影响是非特异性的，独立于其目标miRNA序列，并且每个STTM抑制所有测试的miRNAs。这是STTM技术首次在低等单细胞生物体中进行测试。STTM对miRNA表达的非特异性抑制揭示了不同真核生物中miRNA功能的复杂性。
Short tandem target mimic (STTM) RNAs contain two non-cleavable microRNA (miRNA) binding sites linked by a spacer and can be used to effectively block the functions of specific miRNAs in animals and plants. However, the application of STTMs in unicellular organisms has not been reported. In this study, we tested different STTMs targeting miRNAs of the microalga Chlamydomonas reinhardtii to verify the feasibility of this approach in a unicellular organism. Three previously identifiedC.reinhardtiimiRNAs (miR906-5p, miR1166.1, and miR1150.3) were used as targets and transgenic algae containing STTMs were constructed. All three STTM constructs induced significantly lower miRNA levels, from 84% lower levels of miR1150.3 to 73% lower levels of miR1166.1 compared with non-transgenic controls. Surprisingly, this effect on miRNA levels was non-specific, independent of its target miRNA sequence and each STTM suppressed all miRNAs tested. This is the first time that the STTM technique has been tested in a lower unicellular organism. The non-specific inhibition of miRNA expression by the STTMs revealed the complexity of miRNA functions in diverse eukaryotic organisms.
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
3.1
作者：
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通讯作者：
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