PDMS microchip coated with polydopamine/gold nanoparticles hybrid for efficient electrophoresis separation of amino acids
PDMS microchip coated with polydopamine/gold nanoparticles hybrid for efficient electrophoresis separation of amino acids
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DOI：
10.1002/elps.201100403
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发表时间：
2011-11
期刊：
影响因子：
2.9
通讯作者：
Ruping Liang;Xiang-Ying Meng;Chun-Ming Liu;Jianding Qiu
中科院分区：
文献类型：
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作者：
Ruping Liang;Xiang-Ying Meng;Chun-Ming Liu;Jianding Qiu
本文基于原位化学诱导合成策略，设计并开发了一种新颖、简单、经济和环保的方法，用于用聚多巴胺/金纳米粒子（PDA/Au NPs）修饰聚二甲基硅氧烷（PDMS）微芯片通道，以获得亲水和耐生物污垢的表面。将多巴胺作为还原剂和单体，HAuCl4作为氧化剂触发多巴胺聚合和金属纳米粒子的来源，填充到PDMS微通道中，从而原位生成分布均匀且坚固的PDA/Au NP涂层。扫描电镜和紫外可见光谱证实，Au NPs高度均匀地分散在PDA基体中，且尺寸分布窄。与天然PDMS微通道相比，改性后的表面具有更好的润湿性、高稳定性和抑制电渗透迁移率，减少了对生物分子的非特异性吸附。PDA/Au NP - coating的PDMS微芯片的水接触角和EOF分别为13°和4.17×10−4 cm2/V s，而原生芯片的水接触角和EOF分别为111°和5.33×10−4 cm2/V s。快速高效地分离精氨酸、脯氨酸、组氨酸、缬氨酸和苏氨酸等5种氨基酸，表明PDA/Au NP功能化PDMS微芯片的电泳性能得到了极大的提高。这一步骤为微流控芯片的仿生表面设计提供了一种有效的方法，在高通量和复杂的生物分析中具有前景。
In this paper, a novel, simple, economical and environmentally friendly method based on in situ chemically induced synthesis strategy was designed and developed for the modification of a poly(dimethylsiloxane) (PDMS) microchip channel with polydopamine/gold nanoparticles (PDA/Au NPs) to create a hydrophilic and biofouling resistant surface. Dopamine as a reductant and a monomer, and HAuCl4 as an oxidant to trigger dopamine polymerization and the source of metallic nanoparticles, were filled into the PDMS microchannel to yield in situ a well‐distributed and robust PDA/Au NP coating. Au NPs were highly and uniformly dispersed in/on the PDA matrix with a narrow size distribution, as verified by scanning electron microscopy and UV‐vis spectra. Compared with the native PDMS microchannel, the modified surfaces exhibited much better wettability, high stability and suppressed electroosmotic mobility, and less nonspecific adsorption towards biomolecules. The water contact angle and EOF of PDA/Au NP‐coated PDMS microchip were measured to be 13° and 4.17×10−4 cm2/V s, compared to those of 111° and 5.33×10−4 cm2/V s from the native one, respectively. Fast and efficient separations of five amino acids such as arginine, proline, histidine, valine and threonine suggested greatly improved electrophoretic performance of the PDA/Au NP‐functionalized PDMS microchips. This one‐step procedure offers an effective approach for a biomimetic surface design on microfluidic chips, which is promising in high‐throughput and complex biological analysis.