Fractionation of Urine to Allow Desmosine Analysis by Radioimmunoassay
Fractionation of Urine to Allow Desmosine Analysis by Radioimmunoassay
复制标题
尿液分馏以通过放射免疫分析进行锁链素分析
DOI：
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发表时间：
1992
影响因子：
2.2
通讯作者：
Marti Scott
中科院分区：
文献类型：
--
作者：
B. Starcher;Marti Scott
本研究旨在重新评估尿液中锁链素的放射免疫测定法，该法目前用作弹性蛋白代谢的测量方法。使用离子交换层析、凝胶过滤和亲和层析表明，人尿水解物中至少有五种其他化合物在 RIA 中竞争锁链素。在用丙酮水解之前对尿液进行分级，去除了主要污染物之一。随后可以通过用氯仿/乙醇（60:40）的混合物提取尿液水解产物来去除其他污染物。九份正常成人尿液样本显示，普通尿液中平均 45% 的 RIA 竞争物质不是锁链素。最终提取的残留物保留了所有的锁链素和仅 16% 的原始固体。成人尿液平均含有约 50 pmol 锁链素/mg 肌酸酐，反映每天弹性蛋白的周转量在 3 至 4 mg 之间。
The present study was designed to re-evaluate the radioimmunoassay for desmosine in urine, which is currently used as a measure of elastin metabolism. Using ion exchange chromatography, gel filtration and affinity chromatography it was shown that at least five other compounds in hydrolysates of human urine competed for desmosine in the RIA. Fractionating the urine prior to hydrolysis with acetone removed one of the major contaminants. The other contaminants could subsequently be removed by extracting the urine hydrolysate with a mixture of chloroform/ethanol (60:40). Samples from nine normal adult urines showed that an average of 45% of the RIA competing material in unfractionated urine was not desmosine. The final extracted residue retained all of the desmosine and only 16% of the original solids. The average adult urine contains approximately 50 pmol desmosine/mg creatinine, reflecting a daily turnover of between 3 and 4 mg of elastin per day.
PiZZ α-1-抗胰蛋白酶缺乏症患者体内锁链素（弹性蛋白交联）的尿液排泄，这是一种与肺气肿遗传倾向相关的表型。
DOI：
10.1164/arrd.1985.132.4.821
发表时间：
1985
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Pelham,F;Wewers,M;Crystal,R;Buist,AS;Janoff,A
通讯作者：
Janoff,A