NEUTRALIZATION OF RESPIRATORY SYNCYTIAL VIRUS BY INDIVIDUAL AND MIXTURES OF F-PROTEIN AND G-PROTEIN MONOCLONAL-ANTIBODIES
NEUTRALIZATION OF RESPIRATORY SYNCYTIAL VIRUS BY INDIVIDUAL AND MIXTURES OF F-PROTEIN AND G-PROTEIN MONOCLONAL-ANTIBODIES
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DOI：
10.1128/jvi.62.11.4232-4238.1988
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发表时间：
1988-11-01
影响因子：
5.4
通讯作者：
HIERHOLZER, JC
中科院分区：
文献类型：
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作者：
ANDERSON, LJ;BINGHAM, P;HIERHOLZER, JC
我们确定了几种类型的F和G蛋白单克隆抗体（MAbs）单独或作为混合物对呼吸道合胞病毒（RSV）的中和作用。通过微量中和试验鉴定中和活性，其中通过酶免疫测定法测定病毒复制。完全中和只看到与单克隆抗体对F蛋白。使用针对F蛋白的额外Mab观察到菌株特异性中和、针对RSV的一些菌株的完全中和以及针对其它菌株的无中和。部分中和，病毒复制显著减少但仍然存在，并且用针对F和G蛋白的MAb未观察到中和。在针对F蛋白的所有MAb和针对G蛋白的除三种MAb之外的所有MAb中，观察到两种MAb的混合物的中和增强、中和效力增强或中和滴度增加超过单个MAb。在酶免疫测定中，大多数（10/13）表现出中和活性的单克隆抗体与一些但不是所有的RSV株反应。对应于这10种MAb的表位可能有助于对RSV的中和抗体应答的菌株特异性组分。我们的研究结果表明，解释与单克隆抗体的RSV中和是复杂的，这种中和的研究应包括单克隆抗体和多种RSV株的混合物。
We identified several types of neutralization effected by F and G protein monoclonal antibodies (MAbs) reacted individually or as mixtures against respiratory syncytial virus (RSV). Neutralizing activity was identified by a microneutralization test in which virus replication was determined by enzyme immunoassay. Complete neutralization was seen only with MAbs against the F protein. Strain-specific neutralization, complete neutralization against some strains of RSV, and no neutralization against other strains were seen with an additional Mab against the F protein. Partial neutralization, virus replication significantly reduced but still present, and no neutralization were seen with MAbs against both the F and G proteins. Enhanced neutralization, enhanced efficacy of neutralization, or increased neutralizing titer with a mixture of two MAbs over that for the individual MAbs was seen with all MAbs against the F protein and all but three MAbs against the G protein. Most (10 of 13) of the MAbs that exhibited neutralizing activity reacted with some but not all strains of RSV in an enzyme immunoassay. The epitopes corresponding to these 10 MAbs probably contribute to the strain-specific component of the neutralizing antibody response to RSV. Our results suggest that interpretation of RSV neutralization with MAbs is complex and that studies of such neutralization should include mixtures of MAbs and multiple RSV strains.