The Overlooked Genetic Diversity in the Dryland Soil Surface-Dwelling CyanobacteriumNostoc flagelliformeas Revealed by the Marker GenewspA
The Overlooked Genetic Diversity in the Dryland Soil Surface-Dwelling CyanobacteriumNostoc flagelliformeas Revealed by the Marker GenewspA
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标记基因 wspA 揭示了旱地土壤表层发菜蓝藻中被忽视的遗传多样性
DOI：
10.1007/s00248-020-01610-w
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发表时间：
2020-10-02
影响因子：
3.6
通讯作者：
Yuan, Xiaolong
中科院分区：
文献类型：
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作者：
Gao, Xiang;Xu, Haiyan;Yuan, Xiaolong
生物多样性在旱地生态系统中被认为相对较低。然而，我们可能忽略了这些旱地微种群中积累的遗传变异，这些遗传变异最终会增加旱地生物多样性。在中国西部和西北干旱草原上，存在着两种生活在土壤表层且基因相近的蓝藻，即群落蓝藻（Nostoc communee）和鞭毛蓝藻（Nostoc flagelliforme）。它们分别呈现片状和丝状菌落形状。它们的个体菌落由数百个毛状体和共同的胞外多糖基质组成。N. flagelliformes只分布在旱地，被认为是由N. flagelliformes进化而来。公社。我们以前报道过n。鞭毛菌落非常有限，要么呈圆柱形，要么呈条状。在这篇通讯中，我们进行了标记基因WspA的单核苷酸多态性（SNP）分析以及WspA蛋白inN的系统发育分析。鞭毛群落以深入了解其遗传多样性。SNP分析表明，个体群体中存在着丰富的核苷酸变异，同时这些变异具有一定的进化规律。从克隆的wspas序列中推断出的蛋白质的系统发育分析表明，相对规律的变异可能分散在theN中。不同地区的鞭毛状种群。因此，这些结果提出了一个被低估的遗传多样性隐藏在有限形态背后的旱地蓝藻的场景。也许，我们可以将单个蓝藻菌落视为旱地潜在的生物多样性库。
Biodiversity is recognized to be relatively low in the dryland ecosystem. However, we might overlook the accumulating genetic variation in those dryland micro-populations, which should eventually increase the dryland biodiversity. In the xeric steppes of western and northwestern China, there are two soil surface-dwelling and genetically close cyanobacterial species,Nostoc communeandNostoc flagelliforme. They respectively exhibit lamellate and filamentous colony shapes. Their individual colony is consisted of hundreds of trichomes and the common exopolysaccharide matrix. N. flagelliformeis exclusively distributed in the dryland and supposed to be evolved fromN. commune.We previously reported that the morphological diversity ofN. flagelliformecolonies was very limited, being either cylindrical or strip-like. In this communication, we performed single-nucleotide polymorphism (SNP) analysis of the marker genewspAas well as phylogenetic analysis of the WspA protein inN. flagelliformecolonies to gain insights into its genetic diversity. SNP analysis suggested that there existed plentiful nucleotide variations in the individual colonies and meanwhile these variations shared certain evolutionary regularity. Phylogenetic analysis of the deduced proteins from the clonedwspAsequences suggested that the relatively regular variations were possibly dispersed in theN. flagelliformepopulations of different regions. Thus, these results presented a scenario of the underestimated genetic diversity hidden behind the limited morphotype of dryland cyanobacteria. Maybe, we can consider the individual cyanobacterial colony as a potential biodiversity pool in the drylands.