Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain
Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain
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DOI：
10.1016/j.febslet.2013.01.055
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发表时间：
2013-03-18
期刊：
影响因子：
3.5
通讯作者：
Haga, Hisashi
中科院分区：
文献类型：
--
作者：
Ishihara, Seiichiro;Yasuda, Motoaki;Haga, Hisashi
放射治疗是恶性肿瘤的主要治疗手段之一。然而，照射后存活的细胞通常显示出高水平的侵袭性。这项研究表明，耐辐射肺腺癌表现出高侵袭能力，这取决于肌球蛋白调节轻链（MRLC）的去磷酸化。在胶原凝胶覆盖条件下，低侵袭性肺腺癌亚克隆（A549 P-3）显示圆形形态和MRLC的二磷酸化。与此相反，耐辐射的A549 P-3细胞（A549 P-3 IR）表现出高侵袭性和较低水平的MRLC二磷酸化。此外，抑制MRLC磷酸酶活性可降低侵袭活性。这些结果表明，A549 P-3 IR细胞通过MRLC去磷酸化获得高侵袭力。(C)2013年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Radiotherapy is one of the major treatment modalities for malignancies. However, cells surviving irradiation often display high levels of invasiveness. This study shows that irradiation-tolerant lung adenocarcinoma demonstrates high invasive capability depending on dephosphorylation of the myosin regulatory light chain (MRLC). In a collagen gel overlay condition, low-invasive subclones of lung adenocarcinoma (A549P-3) showed a round morphology and diphosphorylation of MRLC. In contrast, irradiation-tolerant A549P-3 cells (A549P-3IR) displayed high invasiveness and a lower level of MRLC diphosphorylation. In addition, inhibition of MRLC phosphatase activity decreased the invasive activity. These findings suggest that A549P-3IR cells acquire high invasiveness through MRLC dephosphorylation. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.