MOLECULAR PHYLOGENY OF SELECTED SPECIES OF THE ORDER DINOPHYSIALES (DINOPHYCEAE)-TESTING THE HYPOTHESIS OF A DINOPHYSIOID RADIATION
MOLECULAR PHYLOGENY OF SELECTED SPECIES OF THE ORDER DINOPHYSIALES (DINOPHYCEAE)-TESTING THE HYPOTHESIS OF A DINOPHYSIOID RADIATION
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DOI：
10.1111/j.1529-8817.2009.00741.x
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发表时间：
2009-10-01
影响因子：
2.9
通讯作者：
Daugbjerg, Niels
中科院分区：
文献类型：
--
作者：
Jensen, Maria Hastrup;Daugbjerg, Niels
大约80年前，一个基于结构相似性的辐射计划首次为海洋甲藻目（Dinophysiales）提出。这种假设的辐射说明了甲藻属之间的关系，并包括几个独立的，现存的谱系。随后的研究为这些进化谱系提供了形态学和生态学方面的额外信息。我们首次将形态学信息与分子生物学相结合，在现代背景下检验了dinophysioid辐射假说。核编码的LSU rDNA序列，包括D1-D 6结构域，来自27种属于Dinophysis Escherichia b.，鸟尾藻F. Stein，Phalacroma F. Stein，Amphisolenia F. Stein，Cithartes F. Stein和Histoneis F. Stein是从印度洋获得的。以前，LSU rDNA仅从其中之一确定。在Bayesian分析中，Amphisolenia形成了一个很长的基部分支到其他dinophysioids。这些分支成两个独立的谱系，第一个包括具有经典的Phalacroma轮廓的物种，还包括模式种P. porodictyum F。斯坦因此，我们建议恢复Phalacroma属。第二谱系中属之间的关系尚未得到很好的解决。然而，分子系统发育支持单系的Histioneis和Cithatterites和Dinophysis属是多系的，需要一个分类修订。鸟尾丝虫属的物种与Cithaltites同属一组，但这一关系仍未得到解决。系统发育树进一步揭示了甲藻类几个形态特征的趋同进化。根据分子数据，甲藻的进化似乎与先前假设的辐射方案完全不同。4种甲藻与其他已确定的种具有相同的LSU rDNA序列。
Almost 80 years ago, a radiation scheme based on structural resemblance was first outlined for the marine order Dinophysiales. This hypothetical radiation illustrated the relationship between the dinophysioid genera and included several independent, extant lineages. Subsequent studies have supplied additional information on morphology and ecology to these evolutionary lineages. We have for the first time combined morphological information with molecular phylogenies to test the dinophysioid radiation hypothesis in a modern context. Nuclear-encoded LSU rDNA sequences including domains D1-D6 from 27 species belonging to Dinophysis Ehrenb., Ornithocercus F. Stein, Phalacroma F. Stein, Amphisolenia F. Stein, Citharistes F. Stein, and Histioneis F. Stein were obtained from the Indian Ocean. Previously, LSU rDNA has only been determined from one of these. In Bayesian analyses, Amphisolenia formed a long basal clade to the other dinophysioids. These diverged into two separate lineages, the first comprised species with a classical Phalacroma outline, also including the type species P. porodictyum F. Stein. Thus, we propose to reinstate the genus Phalacroma. The relationship between the genera in the second lineage was not well resolved. However, the molecular phylogeny supported monophyly of Histioneis and Citharistes and showed the genus Dinophysis to be polyphyletic and in need of a taxonomic revision. Species of Ornithocercus grouped with Citharistes, but this relationship remained unresolved. The phylogenetic trees furthermore revealed convergent evolution of several morphological characters in the dinophysioids. According to the molecular data, the dinophysioids appeared to have evolved quite differently from the radiation schemes previously hypothesized. Four dinophysioid species had identical LSU rDNA sequences to other well-established species.