Mask, a component of the Hippo pathway, is required for Drosophila eye morphogenesis
Mask, a component of the Hippo pathway, is required for Drosophila eye morphogenesis
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DOI：
10.1016/j.ydbio.2020.05.002
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发表时间：
2020-08-01
影响因子：
2.7
通讯作者：
Johnson, Ruth, I
中科院分区：
文献类型：
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作者：
DeAngelis, Miles W.;McGhie, Emily W.;Johnson, Ruth, I
河马信号是组织大小的重要调节器，但它在组织形态发生中也扮演着鲜为人知的角色。在这里，我们使用果蝇的蛹眼来探索河马效应器yki及其辅助因子面具在形态发生中的作用。我们发现，MASK对于黏附连接的正确分布和积累以及细胞骨架的适当组织是必需的。因此，干扰掩码表达会导致严重的错误图案化，当yki降低或响应异位WTS时，也会观察到类似的缺陷。此外，通过减少MASK表达而产生的图案缺陷被Hippo途径的活性所修饰。RNA测序显示，在眼睛形态发生过程中，MASK需要许多基因的适当表达。这些基因包括与细胞黏附和细胞骨架组织有关的基因，一套全面的促进细胞存活的基因，以及众多的信号转导基因。为了验证我们的转录组分析，我们随后考虑了两个被MASK活性修饰的基因座：FER和VINC，它们已经确立了调节黏附的作用。调节任一位点修饰的掩膜错图化和黏附表型的表达。此外，用yki修饰了FER和VINC的表达。众所周知，河马信号通路对细胞黏附和细胞骨架的变化有反应，但我们的数据表明，河马信号也调节这些结构。
Hippo signaling is an important regulator of tissue size, but it also has a lesser-known role in tissue morphogenesis. Here we use the Drosophila pupal eye to explore the role of the Hippo effector Yki and its cofactor Mask in morphogenesis. We found that Mask is required for the correct distribution and accumulation of adherens junctions and appropriate organization of the cytoskeleton. Accordingly, disrupting mask expression led to severe mis-patterning and similar defects were observed when yki was reduced or in response to ectopic wts. Further, the patterning defects generated by reducing mask expression were modified by Hippo pathway activity. RNA-sequencing revealed a requirement for Mask for appropriate expression of numerous genes during eye morphogenesis. These included genes implicated in cell adhesion and cytoskeletal organization, a comprehensive set of genes that promote cell survival, and numerous signal transduction genes. To validate our transcriptome analyses, we then considered two loci that were modified by Mask activity: FER and Vinc, which have established roles in regulating adhesion. Modulating the expression of either locus modified mask mis-patterning and adhesion phenotypes. Further, expression of FER and Vinc was modified by Yki. It is well-established that the Hippo pathway is responsive to changes in cell adhesion and the cytoskeleton, but our data indicate that Hippo signaling also regulates these structures.