The human T24 Ha-ras1 oncogene: a study of the effects of overexpression of the mutated ras gene product in rodent cells.
The human T24 Ha-ras1 oncogene: a study of the effects of overexpression of the mutated ras gene product in rodent cells.
复制标题
人类 T24 Ha-ras1 癌基因：啮齿动物细胞中突变 ras 基因产物过度表达影响的研究。
作者：
D. Spandidos
将人T24 Ha-ras 1癌基因插入到携带氨基糖苷磷酸转移酶（aph）基因的高表达载体中。通过磷酸钙技术将该重组体引入早期传代啮齿动物细胞中。在遗传霉素的选择压力下，出现了形态改变的细胞灶，这些细胞变得永生化、不依赖锚定和致瘤性。这些细胞过表达T24 Ha-ras 1癌基因的p21产物。结论是p21对这些细胞没有毒性。在重建实验中，其中转化的和正常的细胞以各种细胞密度一起铺板，正常细胞在高细胞密度和不存在选择的情况下抑制转化的表型。
The human T24 Ha-ras1 oncogene was inserted into a high expression vector which carried the aminoglycoside phosphotransferase (aph) gene as a selectable market. This recombinant was introduced into early passage rodent cells by the calcium-phosphate technique. Under the selection pressure of geneticin foci appeared of morphologically altered cells which became immortalized, anchorage independent and tumourigenic. These cells overexpressed the p21 product of the T24 Ha-ras1 oncogene. It was concluded that p21 is not toxic to these cells. In reconstruction experiments, where transformed and normal cells were plated together at various cell densities, normal cells, at high cell density and in the absence of selection, suppressed the transformed phenotype.