Genomic organization, chromosomal localization, and expression of the murine RAB3D gene.
Genomic organization, chromosomal localization, and expression of the murine RAB3D gene.
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小鼠 RAB3D 基因的基因组组织、染色体定位和表达。
DOI：
10.1006/bbrc.2000.3032
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Dickey,BF
中科院分区：
文献类型：
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作者：
Adachi,R;Nigam,R;Tuvim,MJ;DeMayo,F;Dickey,BF
RAB蛋白是RAS小分子GTP酶超家族的成员，在房室间囊泡运输中起着调节作用。交通的每一步似乎都需要至少一个不同的Rab参与，Rab3亚家族参与刺激胞吐。我们报道了我们对小鼠Rab3D基因的研究，该基因是四种哺乳动物Rab3亚型之一。该基因位于13号染色体A2-3区。rab3D基因由5个外显子组成，全长10.6kb，结构基因位于外显子2~5，每个外显子有一个典型的GTP结合基序。Rab3D基因的组织结构与Rab3A相同，但与其他Rab基因不同。Northern印迹分析表明，3‘端非翻译区的交替PolyA+信号可以解释多个转录本的同源性。Rab3D在所有研究的组织中都有表达，主要是在心脏、肺和肝脏，在TATA缺失的启动子区域发现了多种转录因子的结合位点。
Rab proteins, members of the Ras superfamily of small GTPases, play regulatory roles in intercompartmental vesicular transport. Each step of traffic seems to require the participation of at least one distinct Rab, with the Rab3 subfamily involved in stimulated exocytosis. We report our studies on the murine rab3D gene, one of the four mammalian Rab3 isoforms. We located this gene on chromosome 13, region A2–3. The rab3D gene consists of 5 exons spanning 10.6 kb, and the structural gene is contained in exons 2 through 5 with one canonical GTP-binding motif in each exon. Organization of the rab3D gene is identical to that of rab3A but different from other rab genes. Alternative poly-A+signals in the 3′ untranslated region account for the identities of multiple transcripts detected by Northern blot analysis. Rab3D is expressed in all tissues studied, predominantly in heart, lung, and liver, and binding sites for multiple transcription factors are found in the TATA-less promoter region.