Oat β-glucan ameliorates dextran sulfate sodium (DSS)-induced ulcerative colitis in mice
Oat β-glucan ameliorates dextran sulfate sodium (DSS)-induced ulcerative colitis in mice
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DOI：
10.1039/c5fo00563a
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发表时间：
2015-01-01
期刊：
影响因子：
6.1
通讯作者：
Luo, Feijun
中科院分区：
文献类型：
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作者：
Liu, Bo;Lin, Qinlu;Luo, Feijun
溃疡性结肠炎是一种主要的炎症性肠病(IBD)，其特征是通过慢性或复发的免疫系统激活在胃肠道内发生炎症。本研究旨在探讨燕麦β-葡聚糖(βG)对DSS诱导的小鼠结肠炎的保护作用。将80只小鼠随机分为对照组(不给药，不给药)、给药组(只给药)、给药+L-βG组(给药前加500 mg/kgβG)和给药+H-βG组(给药后加1000 mg/kgβG)。与DSS组相比，βG组的临床症状明显减轻，体重减轻、腹泻和结肠缩短，结肠炎的严重程度明显受到抑制，疾病活动指数(DAI)和结肠组织学损害程度明显降低。此外，βG不仅可降低髓过氧化物酶活性(MPO)、一氧化氮(NO)和丙二醛(MDA)水平，而且还可抑制促炎因子如TNF-α、IL-1β、IL-6和iNOS的mRNA和蛋白表达。提示饮食中燕麦βG可能通过抑制促炎因子的表达而发挥抗炎作用。
Ulcerative colitis is a major inflammatory bowel disease (IBD), characterized by inflammation within the gastrointestinal tract through chronic or relapsing immune system activation. The aim of this study is to investigate the potential protective effect of oat beta-glucan (beta G) against colitis induced by DSS in mice. Eighty mice were randomly divided into the control group (no DSS, no beta G), DSS group (DSS only), DSS + L-beta G group (DSS plus 500 mg per kg beta G), and DSS + H-beta G group (DSS plus 1000 mg per kg beta G). Compared with the DSS group, administration of beta G significantly reduced clinical symptoms with less weight loss, diarrhea and shortening of the colon, the severity of colitis was significantly inhibited as evidenced by the reduced disease activity index (DAI) and degree of histological damage in colon. Moreover, treatment with beta G not only decreased myeloperoxidase activity (MPO), and nitric oxide (NO) and malondialdehyde (MDA) levels, but also inhibited mRNA and protein expression of pro-inflammatory factors such as TNF-alpha, IL-1 beta, IL-6 and iNOS. This suggests that oat beta G in diet might exhibit an anti-inflammatory function against colitis through inhibition of expression of pro-inflammatory factors.