Determination of proliferative activity in colorectal carcinoma using monoclonal antibody Ki67
Determination of proliferative activity in colorectal carcinoma using monoclonal antibody Ki67
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DOI：
10.1007/bf02050423
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发表时间：
1997-03-01
影响因子：
3.9
通讯作者：
Gordon, PH
中科院分区：
文献类型：
--
作者：
Kyzer, S;Gordon, PH
目的：在寻找预后判别因素时，使用结直肠癌的 Ki67 免疫反应性来观察该标志物是否与临床和病理参数以及患者的最终预后相关。方法：从 30 例手术切除的腺癌中获取新鲜标本并冷冻在液氮中。用 Ki67 对载玻片进行免疫组织化学染色。检查随机选择的 10 个视野，对每个样本 1,000 个细胞核进行计数。阳性染色细胞核的百分比决定了 Ki67 分数。与Dukes分期、结肠内位置、恶性肿瘤部位、性别、年龄和生存等参数建立相关性。结果：转移性疾病患者的Ki67评分呈正相关，但与Dukes B和C病例无相关性。 Ki67 免疫反应性与病变大小或位置、患者年龄、患者性别，或者患者是否因病死亡或与无病生存五年的患者相比出现复发之间没有相关性。结论：在本研究中，Ki67 免疫反应性与除转移性疾病以外的临床和病理参数没有相关性，并且作为预后判别指标的用途有限。
PURPOSE: In the search for a prognostic discriminant, Ki67 immunoreactivity of colorectal carcinoma was used to see whether this marker correlated with clinical and pathologic parameters and the ultimate prognosis of the patient. METHODS: Fresh specimens from 30 surgically resected adenocarcinomas were obtained and frozen in liquid nitrogen. Slides were immunohistochemically stained with Ki67. Ten randomly chosen fields were examined, and 1,000 nuclei per specimen were counted. The percent positive stained nuclei determined the Ki67 score. Correlation was made with the parameters of Dukes stage, location within the colon, site of malignancy, gender, age, and survival RESULTS: There was a positive correlation of the Ki67 score for patients with metastatic disease, but no correlation was found to Dukes B and C cases. There was no correlation between Ki67 immunoreactivity and size or location of lesion, patient's age, patient's gender, or whether patient died of disease or developed a recurrence compared with those who survived five years disease-free. CONCLUSION: Ki67 immunoreactivity could not be correlated with clinical and pathologic parameters except for metastatic disease in this study and was of limited use as a prognostic discriminant.