Staphylococcus aureus clonal dynamics and virulence factors in children with atopic dermatitis
Staphylococcus aureus clonal dynamics and virulence factors in children with atopic dermatitis
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DOI：
10.1111/j.0022-202x.2005.23916.x
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发表时间：
2005-11-01
影响因子：
6.5
通讯作者：
Kilian, M
中科院分区：
文献类型：
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作者：
Lomholt, H;Andersen, KE;Kilian, M
进行了一项前瞻性队列研究，以确定特应性皮炎儿童1年期间金黄色葡萄球菌定植和感染的克隆动力学，并将特定克隆、辅助基因调节因子（agr）组和毒力因子的产生与湿疹活动相关。11名儿童每6周检查一次，从活动性湿疹、前鼻、腋窝和会阴处取样，并通过特应性皮炎严重程度评分（SCORAD）对湿疹活动性进行评分。个体S.鉴定金黄色葡萄球菌克隆类型，并检查超抗原、毒素的产生，并将其分配到AGR组。S.金黄色葡萄球菌定殖模式的范围从短暂定殖的罕见定殖到通过单个克隆或多达五个克隆的动态交换的持续定殖。生产没有单一的毒力因子，包括超抗原和毒素显着相关的湿疹恶化。在四个孩子中，在殖民克隆的agr组中，访问之间有变化。这些变化与SCORAD值增加19（SE = 7，p = 0.009）相关。属于同一agr组的克隆的变化与较高的SCORAD值无关。在12例两个不同克隆共定殖一个孩子的病例中，有11例克隆属于同一agr组。总之，这一有限的特应性皮炎儿童组显示出高度可变的沙门氏菌定植模式。金黄色葡萄球菌，并且通过使用AGR编码的八肽在菌株之间的交流似乎在体内是有活性的。湿疹的严重程度增加与agr组的变化有关，可能是由于抗原性不同的克隆接管引发的炎症，因为agr组代表了古老的系统发育谱系。
A prospective cohort study was undertaken to determine the clonal dynamics of Staphylococcus aureus colonization and infection during 1 y in children with atopic dermatitis, and to correlate specific clones, accessory gene regulator (agr) groups, and production of virulence factors with eczema activity. Eleven children were examined every 6 wk with swaps taken from active eczema, anterior nose, axillae and perineum, and scoring of eczema activity by severity scoring of atopic dermatitis (SCORAD). Individual S. aureus clonal types were identified and examined for production of superantigens, toxins, and were assigned to agr groups. S. aureus colonization patterns ranged from rare colonization over transient colonization to persistent colonization by a single clone or a dynamic exchange of up to five clones. Production of no single virulence factor including superantigens and toxins was significantly associated with exacerbation of eczema. In four children there was a shift between visits in agr group of colonizing clones. These shifts were associated with an increased SCORAD value of 19 (SE = 7, p = 0.009). Change of clones belonging to the same agr group was not associated with a higher SCORAD value. In 11 of 12 cases with two different clones co-colonizing a child the clones belonged to the same agr group. In conclusion, this limited group of children with atopic dermatitis showed highly variable colonization patterns of S. aureus, and communication between strains by use of agr encoded octa peptides appeared to be active in vivo. Increased severity of eczema was related to a change in agr group and may have been because of inflammation triggered by the takeover of an antigenically different clone, as agr groups represent ancient phylogenetic lineages.