In vitro response of murine alveolar and peritoneal macrophages to Mycobacterium intracellulare.
In vitro response of murine alveolar and peritoneal macrophages to Mycobacterium intracellulare.
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小鼠肺泡和腹膜巨噬细胞对胞内分枝杆菌的体外反应。
DOI：
10.1164/arrd.1983.128.6.1044
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
P. Pratt
中科院分区：
文献类型：
--
作者：
P. Gangadharam;P. Pratt
比较了正常居住和卡介苗激活的雌性瑞士韦氏小鼠的肺泡和腹膜巨噬细胞在体外摄取和随后控制细胞内分枝杆菌增殖的能力。滞留的腹膜巨噬细胞从摄入的那一刻起就无法控制吞噬的细菌的增殖，导致宿主细胞的溶解，而激活的腹膜巨噬细胞和驻留的和激活的肺泡巨噬细胞在可比细菌增殖导致吞噬细胞死亡之前，至少在7天内抑制了细菌的分裂。由于活着的巨噬细胞通常含有数百个分枝杆菌，因此不可能准确确定裂解巨噬细胞所需的细菌数量。每种巨噬细胞的最小细胞内细菌产生时间为20h。两种巨噬细胞之间细菌吞噬速率的差异，无论是驻留的还是激活的，都是巨噬细胞的固有特性，不是由分枝杆菌诱导的，因为在接触乳胶微球后观察到相同的颗粒摄取模式。
Normal resident and BCG-activated alveolar and peritoneal macrophages from female Swiss Webster mice were compared for their ability to ingest and subsequently control the multiplication of Mycobacterium intracellulare in vitro. Resident peritoneal macrophages failed from the moment of ingestion to control the multiplication of engulfed bacilli resulting in host cell lysis, whereas activated peritoneal macrophages and both resident and activated alveolar macrophages constrained bacterial division for at least 7 days before comparable bacterial multiplication led to phagocyte death. The number of bacilli needed to lyse a macrophage was impossible to determine precisely because viable macrophages commonly contained several hundred mycobacteria. Minimal intracellular bacterial generation times were 20 h for each macrophage type. Differences in the rates of bacterial phagocytosis between both macrophage types, either resident or activated, are intrinsic properties of the macrophages and were not induced by the mycobacteria, because the same patterns of particle ingestion were observed after exposure to latex microspheres.