Evolutionary algorithms and flow cytometry to examine the parameters influencing transconjugant formation
Evolutionary algorithms and flow cytometry to examine the parameters influencing transconjugant formation
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DOI：
10.1111/j.1574-6941.2005.00002.x
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发表时间：
2006-01-01
影响因子：
4.2
通讯作者：
Verstraete, W
中科院分区：
文献类型：
--
作者：
Boon, N;Depuydt, S;Verstraete, W
进化算法被用来确定最佳的参数组合的transconjugant形成。作为模型系统，选择gfp标记的TOL质粒pWW0来检查从恶臭假单胞菌到大肠杆菌的转移。流式细胞术结果与平板和显微镜的比较表明，大多数transconjugants是不可培养的。因此，通过流式细胞术测定接合子转移率。进化算法表明，最佳条件是在28摄氏度和最高的营养浓度。这项工作表明，进化算法可以用来找到最佳参数的相互作用，在环境微生物学。
An evolutionary algorithm was used to determine the optimal combination of parameters for transconjugant formation. As a model system, a gfp tagged TOL plasmid pWW0 was chosen to examine transfer from Pseudomonas putida to Escherichia coli. A comparison of flow cytometry results with plating and microscopy showed that the majority of transconjugants were not culturable. The transconjugant ratio therefore was determined by flow cytometry. The evolutionary algorithm showed that the optimal conditions were obtained at 28 degrees C and at the highest nutrient concentrations. This work demonstrates that evolutionary algorithms can be used to find optimal parameter interactions in environmental microbiology.