Expression of tumor necrosis factor-like weak inducer of apoptosis and fibroblast growth factor-inducible 14 in patients with polymyositis and dermatomyositis.
Expression of tumor necrosis factor-like weak inducer of apoptosis and fibroblast growth factor-inducible 14 in patients with polymyositis and dermatomyositis.
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肿瘤坏死因子样弱凋亡诱导剂和成纤维细胞生长因子诱导剂14在多发性肌炎和皮肌炎患者中的表达
DOI：
10.1186/ar4454
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发表时间：
2014-01-27
影响因子：
4.9
通讯作者：
Wang GC
中科院分区：
文献类型：
--
作者：
Peng QL;Shu XM;Tian XL;Lu X;Wang GC
本研究旨在探讨肿瘤坏死因子样弱凋亡诱导因子(TWEY)及其受体成纤维细胞生长因子诱导因子14(Fn14)在多发性肌炎(PM)和皮肌炎(DM)中的表达及其与临床表现的关系。采用双抗体夹心ELISA法检测98例PM/DM患者和37例健康人血清TWE水平。提取36例PM/DM患者和10例健康对照新鲜冻存肌肉组织总RNA，用定量逆转录聚合酶链式反应(RT-PCR)分析TWEEP和Fn14的基因表达水平。对23例PM/DM患者和7例健康对照的肌肉活检标本进行TWEE和Fn14免疫荧光染色。PM/DM患者血清TWEE水平显著低于健康对照组(P <； 0.001)，且与血清CD163水平呈负相关(r = -0.49，P <； 0.001)。PM/DM患者肌肉组织中Fn14m RNA的表达显著高于健康对照组(P <； 0.0 1)，而PM/DM患者肌肉组织中TWE m RNA的表达与正常对照组比较差异无统计学意义(P >； 0.0 5)。肌肉组织中Fn14mRNA水平与肌肉疾病活动度呈正相关(r = 0.512，P <； 0.01)。口咽部吞咽困难组的Fn14mRNA水平显著高于非口咽部吞咽困难组(P< <； )。免疫荧光染色结果显示，23例PM/DM患者中19例TTWE阳性，20例Fn14阳性。健康对照组未检测到TWEY和Fn14的表达。TWEAM-Fn14轴可能参与PM/DM的发病机制。进一步了解TWEEP-Fn14在PM/DM中的作用有助于明确PM/DM的治疗靶点。
The aim of this study was to investigate the expression of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) and its receptor fibroblast growth factor-inducible 14 (Fn14) in patients with polymyositis (PM) and dermatomyositis (DM), and their relation to clinical manifestations. Serum levels of TWEAK were detected in 98 PM/DM patients and 37 healthy controls by using the ELISA method. Total RNA isolated from fresh-frozen muscle tissue samples of 36 PM/DM patients and 10 healthy controls were used for analyzing the mRNA levels of TWEAK and Fn14 by quantitative reverse transcription polymerase chain reaction (RT-PCR). Immunofluorescence staining of TWEAK and Fn14 was conducted on muscle biopsy specimens from 23 PM/DM patients and seven healthy controls. Serum levels of TWEAK were significantly decreased in the PM/DM patients compared to those in the healthy controls (P < 0.001), and serum TWEAK levels negatively correlated with serum CD163 levels in PM/DM patients (r = -0.49, P < 0.001). The expression of Fn14 mRNA was significantly increased in the muscle tissue of PM/DM patients than in the muscle tissue of healthy controls (P < 0.01), whereas the expression of TWEAK mRNA in PM/DM patients was not statistically different from that of the healthy controls (P > 0.05). Fn14 mRNA levels in muscle tissue positively correlated with muscle disease activity (r = 0.512, P < 0.01). Patients with oropharyngeal dysphagia had significantly higher Fn14 mRNA levels than patients without oropharyngeal dysphagia (P < 0.05). The results of immunofluorescence staining showed that 19 out of 23 PM/DM patients were TWEAK-positive, and 20 out of 23 PM/DM patients were Fn14-positive. No detectable expressions of TWEAK or Fn14 were observed in the healthy controls. TWEAK-Fn14 axis may be involved in the pathogenesis of PM/DM. Further understanding of TWEAK-Fn14 function in PM/DM may help to define therapeutic targets for PM/DM.
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影响因子：
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