The significance of Brf1 overexpression in human hepatocellular carcinoma.
The significance of Brf1 overexpression in human hepatocellular carcinoma.
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DOI：
10.18632/oncotarget.6668
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发表时间：
2016-02-02
期刊：
影响因子：
--
通讯作者：
Zhong S
中科院分区：
文献类型：
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作者：
Zhong Q;Xi S;Liang J;Shi G;Huang Y;Zhang Y;Levy D;Zhong S
Brf1(TFIIB相关因子1)在细胞转化和肿瘤发生中起重要作用。然而，Brf1在人肝细胞癌中的表达意义仍有待研究。本研究以人肝细胞癌、小鼠肝肿瘤标本以及正常肝和肿瘤肝细胞系为研究对象，采用细胞学和分子生物学方法检测Brf1基因表达的变化。Brf1在人类肝细胞癌中的表达增加，这与较短的生存时间相关。饮酒的肝细胞癌患者Brf1和Pol III(RNA聚合酶III依赖的)基因转录水平高于非肝细胞癌患者。乙醇对肝癌细胞Brf1和Pol III基因的诱导作用高于非肿瘤细胞。乙醇可以提高细胞转化率。抑制Brf1可抑制酒精促进的细胞转化。饮酒会促进Brf1的表达，从而促进细胞转化。这些研究表明，Brf1是一种新的肝细胞癌生物标志物。
Brf1 (TFIIB-related factor 1) plays a crucial role in cell transformation and tumorigenesis. However, the significance of Brf1 expression in human HCC (hepatocellular carcinoma) cases remains to be addressed. In this study, biopsies of human HCC, liver tumor samples of mice and cell lines of normal and tumor liver were utilized to determine the alteration of Brf1 expression using cytological and molecular biological approaches. Brf1 expression is increased in human HCC cases, which is correlated with shorter survival times. Levels of Brf1 and Pol III (RNA polymerase III-dependent) gene transcription in HCC patients with alcohol consumption are higher than the cases of non-HCC with or without alcohol intake. Induction of Brf1 and Pol III genes by ethanol in hepatoma cells is higher than in non-tumor cells. Ethanol increases the rate of cell transformation. Repression of Brf1 inhibits alcohol-promoted cell transformation. Alcohol consumption enhances Brf1 expression to promote cell transformation. These studies demonstrate that Brf1 is a new biomarker of HCC.