Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35
Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35
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DOI：
10.1042/bj20030452
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发表时间：
2003-09-01
影响因子：
4.1
通讯作者：
Kim, W
中科院分区：
文献类型：
--
作者：
Lee, YM;Kim, W
人驱动蛋白超家族蛋白成员 4 (KIF4) 的大部分在间期与核基质相关，而一小部分则存在于细胞质中。在有丝分裂期间，它在整个过程中都与染色体相关。在本研究中，我们使用酵母双杂交系统、免疫共沉淀和共分级分离鉴定了一种与 KIF4 相互作用的蛋白质。该蛋白是 BRCA2 相关因子 35 (BRAF35)，含有非特异性 DNA 结合高迁移率基团结构域和驱动蛋白样卷曲螺旋结构域。看来两种蛋白质之间的相互作用是通过它们各自的α螺旋卷曲螺旋结构域发生的。联合分级分离实验表明，KIF4 和 BRAF35 存在于约 1 的复合物中。 540 kDa。该复合物的组成和生物学意义值得进一步研究。
A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.